Tag Archives: testosterone

Dynamics of CD68 receptor expression in macrophages of the interstitial space of the rat testis under triptorelin prolonged administration

Ye. V. Stetsuk1*, V. I. Shepytko1, O. Ye. Akimov2,
N. V. Boruta1, M. V. Rud1, L. B. Pelypenko1, O. D. Lysachenko1,
O. V. Vilkhova1, T. A. Skotarenko1, O. V. Voloshyna1

1Department of Histology, Cytology and Embryology,
Poltava State Medical University, Poltava, Ukraine;
2Department of Pathophysiology, Poltava State Medical
University, Poltava, Ukraine;
*e-mail: Stetsuk78@gmail.com

Received: 18 May 2023; Revised: 01 June 2023;
Accepted: 05 June 2023; Available on-line: 20 June 2023

Testosterone, the production of which is stimulated by the release of luteinizing hormone (LH) has a remarkable anti-inflammatory and immunomodulatory effect, and in conditions of testosterone deficiency tissue damage can occur due to excessive differentiation of macrophages into a proinflammatory M1 phenotype. The aim of this study was to determine the spread of CD68 receptors as the marker of inflammation on the cells in the interstitial space and testicular vessels under LH synthesis blockade with tryptorelin. Sexually mature white male rats were randomly divided into control (10 animals) and experimental (25 animals) groups. Animals in the experimental group were injected with a tryptorelin acetate solution (0.3 mg/kg). Immunochemical analysis of CD68+ expression was estimated at Olympus FV10i-LIV laser scanning confocal microscope using fluorescent labeling dye. It was shown that luteinizing hormone deprivation led to an increase in the distribution of the CD68 receptor in the interstitial space and in the testicular vessels from day 30 to 180 of the experiment, associated with the increase of inducible NO synthase activity in testis tissue.

The effect of N-stearoylethanolamine on the lipid composition of the rat testes and testosterone level during the early stages of streptozotocin-іnduced diabetes

O. V. Onopchenko*, T. M. Horid’ko, H. V. Kosiakova,
A. G. Berdyshev, V. M. Klimashevsky, N. M. Hula

Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
*e-mail: onop.89.av@gmail.com

Received: 23 December 2019; Accepted: 27 March 2020

Diabetes is a metabolic disorder with multiorgan complications, including reproductive system dysfunction where lipid imbalance of germ cells play an important role. N-stearoylethanolamine (NSE) shows a modulatory effect on the lipid composition under different pathologies. Therefore, the aim of our study was to investigate the NSE effect on the testes lipid composition and testosterone level in plasma of diabetic rats. Diabetes was induced in Sprague-Dawley rats by a single streptozotocin injection (50 mg/kg). Animals with glucose levels of 8-12 mmol/l were further selected. NSE was administrated to rats (50 mg/kg) for 10 days at 1.5 months after the streptozotocin injection. The rat testes were used for lipid analysis, namely, phospholipid level, fatty acid methyl esters and plasma testosterone estimation. NSE administration to diabetic rats triggered normalization of total and individual phospholipid content, as well as composition of free and phospholipids fatty acids in the rat testes. In addition, the testosterone content showed a slight increase under the action of NSE. Our results showed that the early stages of diabetes caused destructive changes in rat testes that may induce a decrease in future testicular function. NSE administration to diabetic rats normalized the lipid content of rat testes and was correlated with an increased testosterone level. NSE induced the restoration of testes structure and function during the early stages of streptozotocin-іnduced diabetes in rats.

Association of allele variants of receptor gene of androgens (by the number of CAG-repeats) with androgen dependent hormonal metabolic indices of the organism

V. V. Korpachev, S. V. Melnychenko, R. G. Lukashova

State Institution V. Р. Komisarenko Institute of Endocrinology and Metabolism,
National Academy of Medical Sciences of Ukraine, Kyiv;
e-mail: vitascovna@gmail.com

This review discusses up-to-date conceptions concerning an association of androgen receptor gene (AR) allele (by the number of CAG-repeats) variations with the change of the receptor activity in humans. Different contradictory experiment results concerning the AR function dependence on the number of CAG-repeats have been analyzed. The authors discuss this problem paying mostly their attention to the interrelation between the number of CAG-repeats  and indicators of glucose and lipid metabolism in males and females.