Tag Archives: thyroid hormones

Fetuin-A level in patients with untreated thyroid dysfunction

A. S. Idan1*, M. I. Hamzah1, M. S. Khudhair2

1Clinical Biochemistry Department, Al-Nahrain University, Medicine College, Iraq;
2Internal Medicine Department, Al-Nahrain University, Medicine College, Iraq;
*e-mail: alaas3223@gmail.com

Received: 26 August 2023; Revised: 08 November 2023;
Accepted: 01 February 2024; Available on-line: 26 February 2024

Fetuin-A, a plasma glycoprotein, has been demonstrated to play an essential role in the pathogene­sis of several metabolic disorders. This study aimed to estimate fetuin-A serum level in patients with newly diagnosed primary hyperthyroidism (PHT) and subclinical hypothyroidism (SCH) and to examine its correlation with thyroid hormones level, age and sex of patients. The study involved 90 patients with untreated thyroid dysfunction verified with thyroid function test (45 with PHT and 45 with SCH) and 90 control subjects. Triiodo­thyronin (T3), tetraiodothyronin (T4), and thyroid stimulating hormone (TSH) serum concentrations were measured with enzyme-linked fluorescent assay (ELFA), fetuin-A concentration was measured with enzyme-linked immunosorbent assay (ELISA). It was demonstrated that the level of fetuin-A was significantly higher in the PHT group as compared with the control group and showed a significant positive correlation with the T3 level. In the SCH group, the level of fetuin-A was significantly lower and showed a negative correlation with TSH level. Fetuin-A level rose with age in the PHT group and was unaffected by sex in all studied groups. The perfect AUC value obtained for fetuin-A in the comparison between PHT and SCH groups suggests its potential use as a reliable diagnostic marker to differentiate between these two thyroid conditions.

The indices of thyroid system and metabolism of rats under the influence of nanocomposition based on iodine and citrate

R. S. Fedoruk1, U. I. Tesarivska2, I. I. Kovalchuk1, R. Ja. Iskra1,
M.M. Tsap1*, M. I. Khrabko1, O. I. Koleshchuk1

1Institute of Animal Biology, Nаtional Academy of Agrarian Sciences of Ukraine, Lviv;
2State Scientific-Research Control Institute of Veterinary Preparations and Feed Additives, Lviv, Ukraine;
*e-mail: mm_tsap@meta.ua

Received: 05 June 2020; Accepted: 17 May 2021

The search for a new biologically effective iodine compounds that may have practical use and do not cause toxic effects is relevant. The aim of the research was to examine the influence of nanocomposition based on citric acid – iodine helated compex (I2Citr) on the indices of thyroid system activity and metabolism in the blood serum of young male rats. Rats of the experimental groups received I2Citr with water daily for 40 days at a dose of 24 μg I/kg (1st experimental group) and 240 μg I/kg (2nd experimental group) of the body weight. The level of hormones and antibodies in the blood serum was determined with ELISA. Reduced Tg level and triacylglycerols content and elevated  AntiTg,  AntiTPO levels and Ca and albumin content in the serum of rats in both groups compared to control were observed. It was found that in the serum of rats in the 2nd experimental group the levels of T3, T4 and urea, as well as the activity of alkaline phosphatase and AlAT were reduced. No changes in the coefficients of the rats internal organs mass were detected under the action of I2Citr except for the spleen mass, which decreased by about 40% in the rats of 1st group and by 33% – in rats of 2nd group as compared to control. The data obtained indicate modulating influence of I2Citr in a studied doses on thyroid activity without causing toxic effects on animal organism.

Effect of sucralose on the blood content of thyroid hormones

O. Oliynyk1,2*, A. Slifirczyk1, Y. Oliynyk3, B. Pereviznyk3

1Pope John Paul II State School of Higher Education, Biała Podlaska, Poland ;
2Bogomolets National Medical University, Kyiv, Ukraine;
3I. Horbachevsky Ternopil National Medical University, Ukraine;
*e-mail: Alexanderoliynyk8@gmail.com

Received: 22 February 2020; Accepted: 25 June 2020

Sugar substitutes are among the most widely used food additives. Increasing number of scientific research on their adverse effect on various body functions has been appearing lately. The objective of this research is to study the effect of sucralose, a sugar substitute, on the thyroid functional state. Involved in the research were 30 women, aged 19-28, estimating themselves healthy. The blood content of free and general triiodothyronine, free and general thyroxine, as well as of thyrotropic hormone was determined. Thereafter, the women were taking sucralose for a month daily in a dose of 15 mg/kg, the blood content of the hormones mentioned above having been tested again. Reliable 2.0, 1.58, and 1.46 times decrease in the blood content of free and general triiodothyronine, and general thyroxine, respectively, as well as reliable 4.41 times increase in the blood content of thyrotropic hormone (Р < 0.001) has been found. Our findings on the sucralose-induced decrease in the level of thyroid hormones confirm the results of other researchers regarding sucralose as biologically inert compound that should be kept in mind when evaluating its effect on the patients with endocrine pathology.

Biological effects of thyroid hormones

T. S. Saatov, A. A. Abduavaliev

Institute of Bioorganic Chemistry, Uzbekistan Academy of Sciences, Tashkent;
e-mail: t.saatov@yandex.ru

The article presents the findings from the study on multifunctional effects of thyroid hormones in relation to normal and malignantly transformed tissues and cells. Both “rapid” and «slow» effects of thyroid hormones including calorigenic effects and effects over adenylate cyclase – cAMP system have been described.  Thyroxin (Т4) has been established capable to inhibit proliferation and to induce apoptosis of cells carrying Т4 receptors on their membranes as well as to change course of metabolic processes under its effect. Spectrum of Т4 targets is quite broad to include not only cells of hormone-producing organs, to name those of the breast and the colon, but also other types of cells to name melanin-containing ones; Т4 effects resulting in reconstruction of presentation of regulatory proteins on the cell membrane surface to ultimately activate the process of cell apoptosis.  Our findings help determine alternative paths for hormonal regulation of cell proliferation and apoptosis of cells of hormone-dependent tumors, breast cancer, in particular, upon impossibility to regulate the processes by conventional methods. This facilitates understanding mechanisms for activation of signal system of the breast cancer’s cells by hormones upon changes in expression of receptors on the cells’ surface, making possible development of novel strategy for replacement therapy of hormone-dependent tumors upon low efficacy of drug therapy.