Ukr.Biochem.J. 2014; Volume 86, Issue 1, Jan-Feb, pp. 124-130

doi: http://dx.doi.org/10.15407/ubj86.01.124

Activity of antioxidant enzymes of the rat kidneys under mercury dichloride effect

A. Ya. Velyka, V. P. Pishak, I. V. Lopushynska

Bukovinian State Medical University, Chernivtsi, Ukraine;
e-mail: velyka.alla@bk.ru

Salts of heavy metals are excreted by the kidneys and, as  pro-oxidants, stimulate the processes of free radical oxidation. Mercury ions are accumulated in the kidneys. So the study of the features of antioxidant enzymes adaptive response of different kidney layers in response to mercury dichloride is important. Catalase and glytathionperoxidase activity within rat kidneys  72 hours after mercury dichloride intoxication in the ratio of 5 ml per 1 kg of the animal weight was studied. It was important to reveal the influence of the mercury salts on rat kidney antioxidative system. Decreasing glytathionperoxidase activity in cortical and cerebral substances and renal papillae were accompanied by increased contents of oxidative modified proteins and lipids and morphological changes in renal tissue under salt and water loading after mercury dichloride poisoning­. The results obtained evidence for the inhibition of antioxidative protection of enzymes in rat kidneys under the mercury dichloride effect.

Keywords: , , , , ,


References:

  1. Melnichuk DO, Mel’nikova NM, Klikh LV, Zalipukhin OD. Mineral composition of liver and buds of animals venenated by strontium chloride. Modern Probl. Toxocol. 2008;(3):18-20.
  2. Trakhtenberg IM, Chaikovsky YuB, Sokurenko LM. Morphological aspects of pathogenesis of mercury intoxication. Suchasni Problemy Toksykolohii. 2008;(1):11-17.
  3. Yermachenko AI. Proceeding 2d International Medical Congress for students and young scientists, Ternopil, 1998, 168 p.
  4. Goncharuk YeG, Korshun MM. Free radical oxidation as universal non-specific mechanism of damaging action of harmful factors of environment. Zhurn AMN Ukraine. 2004;10(1):131-150.
  5. Matsiopa IV, Grygorieva NP, Meshchyshen IF. Adaptation of antioxidative system in rat kidneys under varying light conditions under tetrachloromethane intoxication and melatonin influence. Ukr Biokhim Zhurn. 2010 Mar-Apr;82(2):75-84. PubMed
  6. Pishak VP, Bilooky VV, Rogovy YuE. Versatility damage of proximal tubule upon kidney deseases. Clin Exp Pathol. 2005;4(1):72-76.
  7. Gerush IV, Grygorieva IV, Meshchyshen IF, Matsiopa IV. Free radical mechanisms for trichloromethane toxic lesions and their correction by Echinacea purpurea and melatonin drugs. Chernivtsi: Bukovinian State Medical University, 2012. 180 p.
  8. Pishak VP, Velikaia AYa, Matsepa IV. Changes of indexes of lipid peroxidation and proteins in rat kidneys in case of water and salt loading. Ukr Zhurn Klin Lab Med. 2011;6(4):38-40.
  9. Gozhenko AI, Rogovy YuE, Fedoruk OS. “Hidden” damage to the proximal segment of nephron. Odes Med Zhurn. 2001;(5 (67)):16-19.
  10. Vladimirov YuA, Archakov AI. Lipid peroxidation in biological membranes. Moscow: Nauka, 1972. 252 p. (in Russian).
  11. Meshchyshen IF. Method for determining the oxidative modification of blood plasma(serum) proteins. Buk Med Herald. 1998;2(1):156-158.
  12. Koroliuk MA, Ivanova LI, Mayorova IG, Tokarev VE. A method of determining catalase activity. Lab Delo. 1988;(1):16-9. Russian. PubMed
  13. Gerush IV, Grygorieva IV, Meshchyshen IF. Rationalization proposal of Chernivtsi State Medical University No.25/95.
  14. Venerucci F. Histopathology kits: methods and applications. Bologna, Milan: Bio-Optica, 2001. 95 p.
  15. Oivin IA. Statistical evaluation of the results of experimental investigations. Patol Fiziol Eksp Ter. 1960 Jul-Aug;4:76-85. Russian.  PubMed
  16. Gozhenko AI, Sluchenko AN. Functional state of the kidneys under conditions of water and salt loads in pregnant rats against the background of sublimate nephropathy. Nephrology. 2006;10(1):72-76.
  17. Goncharova LV, Kuzmenko IA. Osmoregulating function of kidneys at toxic nephropathies caused by carbon tetrachloride. Actual Probl. Transp. Med. 2006;(2(4)):43-47.
  18. Matsiopa IV, Meshchyshen IF, Davydenko IS. Morphological features of rat kidneys upon experimental hepatitis at the combined action of NEP and melatonin  under artificial equinox. World Med. Biol. 2008;(4):50-54.
  19. Velikaya AYa, Pishak VP. Matsepa IV. Natural resources, biodiversity and prospects for science education. International Sci-pract. Conference: Abstracts, Omsk, 2012, 165-167.
  20. Velyka AYa, Pishak VP, Matsiopa IV. Changes of the indices of thiobarbiturate-reactive products in the rat blood under water loading conditions with underlying sublimate nephropathy. Buk Med Herald. 2012;16(3(63)):30-32.

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License.