Ukr.Biochem.J. 2013; Volume 85, Issue 4, Jul-Aug, pp. 94-97


Distribution of 5-fluorouracil between lymphocytes and blood plasma

M. A. Stashkevych, E. V. Khomutov, O. P. Shatova, Yu. V. Dumanskiy, I. I. Zinkovych

M. Gorky Donetsk National Medical University, Ukraine;

In blood plasma of 8 healthy volunteers with resuspended lymphocytes incubated with 5-fluorou­racil (5-FU) the drug distribution between cells and liquid was assessed by means of HPLC. Rapid accumulation of 5-FU in lymphocytes was proved (the drug concentration on the 3-rd minute is 2.5-fold higher than in plasma) as well as the absence of temporal changes of 5-FU content both in lymphocytes and blood plasma during 30 minutes of experiment.

Keywords: , , ,


  1. Ceilley RI. Mechanisms of action of topical 5-fluorouracil: review and implications for the treatment of dermatological disorders. J Dermatolog Treat. 2012 Apr;23(2):83-9. Review. PubMed, CrossRef
  2. Patel K, Anthoney DA, Crellin AM, Sebag-Montefiore D, Messruther J, Seymour MT. Weekly 5-fluorouracil and leucovorin: achieving lower toxicity with higher dose-intensity in adjuvant chemotherapy after colorectal cancer resection. Ann Oncol. 2004 Apr;15(4):568-73. PubMed, CrossRef
  3. Bondar’ GV, Kondratiuk BP, Komendant VV, Gonchar AG. Endolymphatic polychemotherapy in comprehensive treatment of pulmonary cancer after a radical surgery. Klin Khir. 1999;(1):18-20. Russian. PubMed
  4. Yakovets IuI, Kondratyuk BP, Popovich AIu. Complex treatment of patients with non-small-cell lung cancer (NSCLC).  Surgery Ukraine. 2005;13(1):106-109.
  5. Bondar GV, Popovich AYu, Lisovskaya NYu, Popovich YuA. Contemporary capabilities of chemotherapy for lung cancer. Int Med J. 2006;(2):82-86.
  6. Patent RU 219528, ICP A61K31/00, A61P35/00.  A method for treatment of endometrial cancer / Sidorenko Yu.S., Pustovalova A.V., Nerodo G.A. Publ. 27.12.2002, No. 2000110761/14.
  7. Colville H, Dzadony R, Kemp R, Stewart S, Zeh HJ 3rd, Bartlett DL, Holleran J, Schombert K, Kosovec JE, Egorin MJ, Beumer JH. In vitro circuit stability of 5-fluorouracil and oxaliplatin in support of hyperthermic isolated hepatic perfusion. J Extra Corpor Technol. 2010 Mar;42(1):75-9. PubMedPubMedCentral
  8. Au JL, Su MH, Wientjes MG. Extraction of intracellular nucleosides and nucleotides with acetonitrile. Clin Chem. 1989 Jan;35(1):48-51. PubMed
  9. Mackey JR, Galmarini CM, Graham KA, Joy AA, Delmer A, Dabbagh L, Glubrecht D, Jewell LD, Lai R, Lang T, Hanson J, Young JD, Merle-Béral H, Binet JL, Cass CE, Dumontet C. Quantitative analysis of nucleoside transporter and metabolism gene expression in chronic lymphocytic leukemia (CLL): identification of fludarabine-sensitive and -insensitive populations. Blood. 2005 Jan 15;105(2):767-74. PubMed, CrossRef
  10. Molina-Arcas M, Bellosillo B, Casado FJ, Montserrat E, Gil J, Colomer D, Pastor-Anglada M. Fludarabine uptake mechanisms in B-cell chronic lymphocytic leukemia. Blood. 2003 Mar 15;101(6):2328-34. PubMed, CrossRef
  11. Lang TT, Selner M, Young JD, Cass CE. Acquisition of human concentrative nucleoside transporter 2 (hcnt2) activity by gene transfer confers sensitivity to fluoropyrimidine nucleosides in drug-resistant leukemia cells. Mol Pharmacol. 2001 Nov;60(5):1143-52. PubMed
  12. Deschoolmeester V, Baay M, Van Marck E, Weyler J, Vermeulen P, Lardon F, Vermorken JB. Tumor infiltrating lymphocytes: an intriguing player in the survival of colorectal cancer patients. BMC Immunol. 2010 Apr 12;11:19. PubMed, PubMedCentralCrossRef
  13. Leichman CG. Schedule dependency of 5-fluorouracil. Oncology (Williston Park). 1999 Jul;13(7 Suppl 3):26-32. Review. PubMed
  14. Milano G. Fluoropurimidines in cancer therapy. Totowa, New Jersey: Humana press, 2003. P. 29-36.
  15. Jiang H, Lu J, Ji J. Circadian rhythm of dihydrouracil/uracil ratios in biological fluids: a potential biomarker for dihydropyrimidine dehydrogenase levels. Br J Pharmacol. 2004 Feb;141(4):616-23. PubMed, PubMedCentral, CrossRef
  16. Raida M, Kliche KO, Schwabe W, Häusler P, Clement JH, Behnke D, Höffken K. Circadian variation of dihydropyrimidine dehydrogenase mRNA expression in leukocytes and serum cortisol levels in patients with advanced gastrointestinal carcinomas compared to healthy controls. J Cancer Res Clin Oncol. 2002 Feb;128(2):96-102. PubMed, CrossRef
  17. Katayanagi S, Aoki T, Takagi Y, Ito K, Sudo H, Tsuchida A, Koyanagi Y. Measurement of serum thymidine phosphorylase levels by highly sensitive enzyme-linked immunosorbent assay in gastric cancer. Oncol Rep. 2003 Jan-Feb;10(1):115-9. PubMed, CrossRef

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License.