Ukr.Biochem.J. 2013; Volume 85, Issue 3, May-Jun, pp. 22-30


Effect of glyphosate on the energy exchange in carp organs

A. A. Zhidenko, E. V. Bіbchuk, E. V. Barbukho

Taras Shevchenko Chernihiv State Pedagogical University, Ukraine;

The use of glyphosate as a herbicide in agriculture can lead to the presence of its residues and metabolites (aminomethylphosphonic acid) in food for human consumption and pose a threat to health. The effect of these herbicides on the fish organism at the biochemical level has been insufficiently studied. We studied changes in the content of adenine nucleotides, enzyme activity, quantitative indexes of energy metabolism substrates in carp under the action of glyphosate. It has been found that proteins are the major ener­gy substrate under the influence of glyphosate in the liver, brain, white muscle of carp yearlings. Glyphosphate  decreases energy metabolism in the brain of carp and increases it in the white muscles. The growth of activity of catabolic enzymes in the liver under the influence of glyphosate can be attributed to the adaptive remodelling of metabolic pathways for homeostasis and enantiostasis in response to herbicides

Keywords: , , , , , , , ,


  1. Addition to the list of pesticides and agrochemicals permitted for use in Ukraine. Ministry of Environmental Protection of Ukraine, Kyiv, 2009. 304 p.
  2. Kuznetsova EM, Chmil VD. Glyphosate: environmental fate and levels of residues. Sovrem Probl Toksikol. 2010;(1):87-95.
  3. Plant protection products and fertilizers permitted for use in the Republic of Belarus (for entities) Herbicides [electronic resource].  State Register of plant protection products (pesticides) and fertilizers permitted for use on the territory of the Republic of Belarus. 2011. Regime of access:, 156 p.
  4. Guidelines for determination of trace amounts of pesticides in food, feed and the environment. Kyiv: National Agrarian University of Ukraine, Lab. of  Quality and Safety of Agricultural Products, 2004;(39): 252 p.
  5. Filippovich YuB, Egorova TA, Sevastyanova GA. General chemistry: Practical works / Ed. Yu. B. Filippovich. M.: Prosveshchenie, 1982. 318 p.
  6. Yavonenko AF, Yakovenko BV, Grubinko VV, Zhydenko AO. The dependence of the survival of juvenile carp in winter time on the content of free amino acids and proteins in the muscles of fish. Fisheries. 1989;(43):24-29.
  7. Ronin VS, Starobinets GM, Utevskiy NL. Practical guide for training in the methodology of clinical laboratory tests. M.: Meditsina, 1977. 335 p.
  8. Skulachev VP. The ratio of oxidation to phosphorylation in the respiratory chain. M.:  Academy of Sciences of the USSR, 1962. 156 p.
  9. Lowry OH, Lopez SA. The determination of inorganic phosphate in the presence of labile phosphate esters. J Biol Chem. 1946 Mar;162:421-8. PubMed
  10. Schachman HK. Ultracentrifugation in Biochemistry. New York: Acad. Press., 1959. 356 p.
  11. Casey CA, Anderson PM. Subcellular location of glutamine synthetase and urea cycle enzymes in liver of spiny dogfish (Squalus acanthias). J Biol Chem. 1982 Jul 25;257(14):8449-53. PubMed
  12. Zinich VN. A method of determining 2-oxoglutarate dehydrogenase activity in intact mitochondria. Ukr Biokhim Zhurn. 1986 Mar-Apr;58(2):73-7. Russian. PubMed
  13.  L’vova SP. Phosphorylase and glucose-6-phosphatase activity in rat tissues during ontogenesis. Ukr Biokhim Zhurn. 1985 Jan-Feb;57(1):36-41. Russian. PubMed
  14. Mekhed OB, Iakovenko BV, Zhydenko AO. Effect of zenkor on the amount of glucose and activity of gluconeogenesis enzymes in carp’s (Cyprinus carpio L.) tissues at different temperatures. Ukr Biokhim Zhurn. 2004 May-Jun;76(3):110-3. Ukrainian. PubMed
  15. Biochemica information.  W. Germany: Boehringer Manneheim GmbH, Biochemica, 1975. Bd.1. 184 р.
  16. Biochemica information. W. Germany: Boehringer Manneheim GmbH, Biochemica, 1975. Bd.2. 167 p.
  17. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265-75. PubMed
  18. Modern methods in biochemistry. Ed. V.N. Orekhovich. M.: Meditsina, 1964. 344 p.
  19. Malyarevskaya AYa, Bilyk TI. Standard methods for the study of productivity of fish species within their habitats. Part V. Vilnius: Institute of Zoology and Parasitology,  Academy of Sciences of the Lithuanian SSR, 1985;83-89.
  20. Kudelin BK, Kaminskiy IuL, Ivanova IF, Gavrilin SS. Fractionation of nucleosides and nucleoside-5′-mono-, di and triphosphates by thin layer chromatography in silufol. Biokhimiia. 1979 Feb;44(2):368-71. Russian. PubMed
  21. Malyarevskaya AYa, Bilyk TI, Sherstyuk VV, Tugay VA. Seasonal dynamics of macro-erg compounds in fish muscle.  Gydrobiol Zhurn. 1985; 21(4):55-62.
  22. Gosh RI. Energy metabolism of gonial cells and embryos in fish. K.: Naukova dumka, 1985. 146 p.
  23. Yarzhombek AA, Limanskiy VV, Scherbina TV. et al. Handbook of fish physiology. Ed. A.A. Yarzhombek. M.: Agropromizdatelstvo, 1986. 192 p.
  24. Smirnov LP. Lipids in physiological and biochemical adaptations of ectothermic organisms to abiotic and biotic environmental factors. L.P. Smirnov, V.V. Bogdan. M.: Nauka, 2007. 182 p.
  25. Mischenko TV. Separate indexes of carp lipid exchange as herbicides intoxication indicators. Sci. Notes Ternopil Nat. Pedag. Univ. named after Volodymyr Hnatiuk. Series: Biology.  2008;(3(37)):114-117.
  26. Creac’h Y. Protein thiols and free amino acids of tissues in the carp (Cyprinus carpio L.) during prolonged fasting. Arch Sci Physiol (Paris). 1966;20(1):115-21. French. PubMed
  27. Bibchuk KV, Zhydenko AA. Current problems of aquatic ecosystems: theses of reports of All-ukrainian scientific practical conference (Dnipropetrovsk, 18 october 2007). – Dnipropetrovsk: Vydavnytstvo of Dnipropetrovsk National University, 2007; 6-7.
  28. Kurant VZ. The role of protein metabolism in the fish  adaptation to the effect of heavy metal ions: Avtoref. dis. … dokt. biol. nauk.  Kyiv, 2003. 38 p.
  29. Zhydenko AA, Kovalenko EM. The Influence of Roundup on the dynamics of histological changes in organs of carps. Gydrobiol Zhurn. 2006;42(6):104-111.

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License.