Ukr.Biochem.J. 2020; Volume 92, Issue 1, Jan-Feb, pp. 103-112


Biochemical indicators of green photosynthetic bacteria Chlorobium limicola response to Cu(2+) action

T. B. Sehin1, S. O. Hnatush1, O. D. Maslovska1,
A. A. Halushka1, Y. H. Zaritska2

1Ivan Franko National University of Lviv, Ukraine;
2State Scientific-Research Control Institute of Veterinary
Medicinal Products and Feed Additives, Lviv, Ukraine;

Received: 11 March 2019; Accepted: 29 November 2019

Photolithotrophic sulfur bacteria are involved in  biota functioning and have  a biotechnological potential for bioremediation of contaminated environment, but the mechanisms of xenobiotics, in particular of heavy metal ions damaging action and the pathways of photolithotrophic bacteria adaptation  under these conditions have not been established. In this work, the biochemical indicators of green photosynthetic bacteria Chlorobium limicola response to Cu ions were studied. C. limicola cells were incubated during one hour in buffer containing copper (II) sulfate in 0.05–0.5 mM concentrations and grown for 8 days in GSB medium. The content of Cu2+ in cells was estimated by atomic absorption spectroscopy. The activity of enzymes of antioxidant defense, photosynthetic pigments and glutathione content, indexes of lipids unsaturation and membrane viscosity as markers of  membrane fluidity were estimated. It was shown that the response of green photosynthetic bacteria C. limicola to Cu2+ action varied  depending on cations concentration. Under the influence of metal salt at 0.05 mM concentration, the activity of antioxidant enzymes, GSH/GSSG ratio, the content of photosynthetic pigments and membrane fluidity indexes were higher as compared with control. Under the increase of copper (II) sulfate concentration to 0.25 mM, the activity of antioxidant  enzymes was lower compared to the response of the cells under the influence of 0.05 mM copper (II) and the GSSG content was increased.  Under the influence of 0.5 mM copper (II) the indexes of membrane fluidity did not differ from the control, but superoxide dismutase and peroxidase activity inhibition and  the further decrease of GSH/GSSG ratio were observed followed by the highest Cu2+ cations accumulation in cells and significant decrease of  bacteria biomass growth.

Keywords: , , , ,


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