Ukr.Biochem.J. 2022; Volume 94, Issue 5, Sep-Oct, pp. 59-68


Changes in the activity of phosphatases, calcium and phosphorus in rats with the different courses of gingivitis under correction by anti-inflammatory gel

O. Avdeev1, R. Drevnitska2, N. Gevkaliuk1,
Yu. Bandrivsky1*, A. Boykiv3

1Department of Pediatric Dentistry, I. Horbachevsky Ternopil National
Medical University, Ternopil, Ukraine;
2Department of Dental Therapy, I. Horbachevsky Ternopil National
Medical University, Ternopil, Ukraine;
3Department of Orthopedic Dentistry, I. Horbachevsky Ternopil National
Medical University, Ternopil, Ukraine;

Received: 27 January 2022; Revised: 20 March 2022;
Accepted: 14 October 2022; Available on-line: 19 December 2022

The aim of the study was to evaluate changes in the activity of acid and alkaline phosphatases, calcium and phosphorus levels in rats with different courses of experimental gingivitis upon treatment with anti-inflammatory gel with Neovitin and peptide complexes. The experiment was conducted on 100 white nonlinear male rats aged 5-6 months divided into 10 groups: 1 control and 9 – with different courseі of gingivitis. The activity of alkaline and acid phosphatase (ALP, ACP), the levels of calcium (Ca) and phosphorus (P) in rat blood serum and gingiva supernatant were determined. It was found that upon gingivitis, the activity of ALP in blood serum decreased and in gingiva supernatant increased in all groups of animals compared to the control group. The activity of ACP in the serum decreased in hypoergic and hyperergic animal groups and increased in normergia, and in gingiva supernatant increased in all groups: by 2 times in normoergic and hypoergic animals and by 1.4 times in hyperergic. The treatment with anti-inflammatory gel normalized the activity of ALP in both serum and supernatant and decreased the ACP activity in the serum of animals in hypo- and hyperergic groups. The content of serum Ca increased in all groups, and in the supernatant of the gingiva even exceeded the control value. The content of phosphorus in the supernatant of periodontal tissues decreased. The development of the inflammatory process in the periodontium of rats with gingivitis was accompanied by changes in the activity of ACP, ALP, the content of Ca and P in the blood serum and gingival supernatant. The treatment with gel containing neovitin and peptide complexes had a more pronounced therapeutic effect in rats with unchanged reactivity of the organism.

Keywords: , , , ,


  1. Health Organization, 2020. Oral health.
  2. Petersen PE, Bourgeois D, Ogawa H, Estupinan-Day S, Ndiaye C. The global burden of oral diseases and risks to oral health. Bull World Health Organ. 2005;83(9):661-669. PubMed, PubMedCentral
  3. Kostura VL, Bezvushko EV. Prevalence and structure of periodontal tissue diseases in overweight children. Clin Dentistry. 2017;(2):42-47. (In Ukrainian). CrossRef
  4. Klitynska OV, Melnyk VS, Stypnicka EM. Analysis of the parodontal tissue in school age patients in Uzhgorod area. Ukr Med Almanac. 2012;15(3):89-90. (In Ukrainian).
  5. Loos BG, Papantonopoulos G, Jepsen S, Laine ML. What is the Contribution of Genetics to Periodontal Risk? Dent Clin North Am. 2015;59(4):761-780. PubMed, CrossRef
  6. van der Veen MH, Volgenant CMC, Keijser B, Ten Cate JBM, Crielaard W. Dynamics of red fluorescent dental plaque during experimental gingivitis – A cohort study. J Dent. 2016;48:71-76. PubMed, CrossRef
  7. Marsh PD, Zaura E. Dental biofilm: ecological interactions in health and disease. J Clin Periodontol. 2017;44(Suppl 18):S12-S22. PubMed, CrossRef
  8. Zemouri C, Jakubovics NS, Crielaard W, Zaura E, Dodds M, Schelkle B, Loos BG. Resistance and resilience to experimental gingivitis: a systematic scoping review. BMC Oral Health. 2019;19(1):212. PubMed, PubMedCentral, CrossRef
  9. Beloklitskaya GF, Gorgol KO. Leading local risk factors in the development of inflammatory periodontal disease in young people. Dentistry. Aesthetics. Innovation. 2017;1(2): 203-214. (In Russian).
  10. Chereda VV. The influence of dental status on the risk of inflammatory periodontal diseases in addlescents. Actual problems of modern medicine: Bull Ukr Med Stomatol Acad. 2013;3(43):74-77. (In Ukrainian).
  11. Bamashmous S, Kotsakis GA, Kerns KA, Leroux BG, Zenobia C, Chen D, Trivedi HM, McLean JS, Darveau RP. Human variation in gingival inflammation. Proc Natl Acad Sci USA. 2021;118(27):e2012578118. PubMed, PubMedCentral, CrossRef
  12. 12. Gevkalyuk N, Sidlyaruk N, Pynda M, Pudiak V, Krupey V. Condition of non-specific resistance of oral mucous membrane in children with viral influenza stomatitis in the concept of MALT-system. Georgian Med News. 2018;(280-281):34-40. (In Russian). PubMed
  13. Avdeev AV, Boykiv A, Drevnitskaya R. Changes in the indicators of lipid peroxidal oxidation and antioxidant system in the serum of the blood in animals with experimental periodontitis with changed reactivity. Georgian Med News. 2019;(287):124-127. (In Russian). PubMed
  14. Avdeev ОV, Drevnitska RO, Boykiv AB, Vydoinyk OYa. Condition of fagocytosis of experimental animals with periodontitis due to modified reactivity. Wiad Lek. 2019;72(3):401-404. PubMed
  15. Bandrivsky Y, Bandrivska O, Gnid R, Minko L, Shevchuk M. Indicators of markers of bone metabolism in patients with generalized periodontitis depending on blood group. Arch Balk Med Union. 2019;54(1):72-77. CrossRef
  16. Bhattacharjee R, Nekkanti S, Kumar NG, Kapuria K, Acharya S, Pentapati KC. Efficacy of triphala mouth rinse (aqueous extracts) on dental plaque and gingivitis in children. J Investig Clin Dent. 2015;6(3):206-210. PubMed, CrossRef
  17. Borodenko DI, Chumakova YuG. Evaluation of therapeutic and preventive action of the injection form of polypeptides in the experiment on the model of periodontitis in rats. Bull Dentistry. 2019;32(2): 9-14. (In Ukrainian). CrossRef
  18. Bandrivsky YL, Bandrivska OO, Shkrebnyuk RY, Dyryk VT. Prevalence of the generalized periodontitis in patients with different groups blood in depending on age and periodontal biotype. Wiad Lek. 2020;73(1):119-122. PubMed
  19. Bandrivsky Y, Bandrivska O, Malko N, Vydoinyk, O, Iskiv M. The effectiveness of the use of polypeptide drugs and their effect on the metabolic parameters of oral fluid in patients with generalized periodontitis in depending on blood type. Pharmacia. 2022;69(2):429-435. CrossRef
  20. Pat. 65771 UA, ICP G09B 3/28. Method of modeling periodontitis. Machogan VR, Avdeev OV. Publ. 12.12.2011, Bul. № 23. (In Ukrainian).
  21. Pat. 146656 UA, ICP G09B 23/28. Method of modeling gingivitis with hypoergic course of inflammatory reaction. Drevnitskaya RO, Avdeev OV, Boykiv AB. Publ. 10.03.2021, Bul. № 10. (In Ukrainian).
  22. Pat. 146655 UA, ICP G09B 23/28. Method of modeling gingivitis with hyperergic course of inflammatory reaction. Drevnitskaya RO, Avdeev OV, Boykiv AB. Publ. 10.03.2021, Bul. № 10. (In Ukrainian).
  23. Bandrivskaia NN, Mrochko OI, Bandrivskiy IuL. Physical, biochemical and bacterioscopic parameters of mouth lavage in patients with periodontium diseases and working on ethanol production enterprises. Med Tr Prom Ekol. 2014;(5):31-34. (In Russian). PubMed
  24. Ansari S, Ito K, Hofmann S. Alkaline Phosphatase Activity of Serum Affects Osteogenic Differentiation Cultures. ACS Omega. 2022;7(15):12724-12733. PubMed, PubMedCentral, CrossRef
  25. Mehić B. Bioethical Principles of Biomedical Research Involving Animals. Bosn J Basic Med Sci. 2011;11(3):145-146.
  26. Lopach SN, Gubenko AV, Babich PN. Statistical methods in medical and biological research Excel. K.: Marion, 2001. 410 p. (In Russian).
  27. Eastell R, Blumsohn A. The value of biochemical markers of bone turnover in osteoporosis. J Rheumatol. 1997;24(6):1215-1217. PubMed
  28. Drevnitskaya RO, Gevkalyuk NO, Avdeev OV. Dynamics of phosphatase activity in blood serum and periodontal tissue homogenate in experimental gingivitis. Clin Dentistry. 2020;(3):71-77. (In Ukrainian). CrossRef

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