Category Archives: Uncategorized
XIІ Ukrainian Biochemical Congress (30 September – 4 October 2019, Ternopil)
Kosterin S.O., Matyshevska O.P., Danilova V.M., Grigorieva M.V.
Allelic polymorphisms of DNA repairing genes as markers of resistance to asbestos-containing aerosols
T. A. Andrushchenko1, S. V. Goncharov2, V. E. Dosenko2,
D. O. Stroy2, K. E. Ishchejkin3
1SI “Kundiiev Institute of Occupational Health of the National Academy of Medical Sciences of Ukraine”, Kyiv;
2Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv;
3Ukrainian Medical Stomatological Academy, Poltava;
e-mail: imp-cys@ukr.net
Received: 14 March 2019; Accepted: 18 October 2019
We studied the frequency distribution of DNA repairing genes allelic polymorphisms in the occupational group of asbestos-cement plants workers (n = 95). The aim of the work was to determine the probable molecular genetic markers of resistance to the development of bronchopulmonary pathology under the action of chrysotile-asbestos-containing dust. In 46 workers with chronic forms of bronchopulmonary pathology and 49 workers of the same professions without chronic diseases of the respiratory system, allelic polymorphisms XPD (rs13181, rs799793), ERCC1 (rs11615), XRCC3 (rs861539), XRCC1 (rs25487), ATM (rs664677), XRCC7 (rs7003908) and MLH1 (rs1799977) were determined using the real-time polymerase chain reaction. It was established that XRCC1•G/A (rs25487) (OR = 0.45; 95% CI: 0.18–1.10; P = 0.050; χ2 = 3.73); MLH1•A/A (rs1799977) (OR = 0.28; 95% CI: 0.14 – 0.71; P = 0.003; χ2 = 8.75) genotypes contribute to the resistance to bronchopulmonary pathology development, while XPD•Asn/Asn (rs799793) (OR = 2.20; 95% CI: 1.75–2.77; P = 0.001; χ2 = 6.62); XRCC1•A/A (rs25487) (OR = 1.73; 95% CI: 1.23–2.43; P = 0.040; χ2 = 3.92); ATM•T/T (rs664677) (OR = 3.47; 95% CI: 1.01–12.51; P = 0.020; χ2 = 4.98); MLH1•A/G (rs1799977) (OR = 2.95; 95% CI: 1.17–7.49; P = 0.010; χ2 = 6.42) genotypes were found to be associated with the risk of respiratory disease development. The obtained results show interconnection between certain alleles of DNA repair genes and the risk of bronchopulmonary pathology development under the influence of industrial aerosols, including asbestos-containing ones.
Corvitin modulates the content of lipids in rat liver bile
T. V. Vovkun1, P. I. Yanchuk1, L. Ya. Shtanova1,
S. P. Veselsky1, N. B. Filimonova1, I. V. Komarov2
1ESC “Institute of Biology and Мedicine”, National Taras Shevchenko University of Kyiv, Ukraine
2“Institute of High Technologies”, National Taras Shevchenko University of Kyiv, Ukraine;
e-mail: shtanova@ukr.net
Received: 23 March 2019; Accepted: 18 October 2019
Quercetin (QUE) and its water-soluble form сorvitin are medicinally important members of the flavonoid family and the most prominent dietary antioxidants. Numerous pharmacological effects of quercetin include protection against diseases, such as atherosclerosis, myocardial infarction, and cerebrovascular diseases. Corvitin modulates liver blood flow but its effects on liver lipid metabolism have not been understood. We investigated the influence of corvitin (2.5, 5, 10 mg/kg) on the formation and secretion of cholesterol (Chol), cholesterol esters (EChol), phospholipids (PLs), free fatty acids (FFAs), and triglycerides (TGs) into the bile. Secreted bile was collected during 2.5 h of the experiment. Lipid fractions of the bile were separated by thin-layer chromatography. We defined that administration of corvitin caused a significant (P < 0.05–0.001) increase in levels of all studied lipid components of bile. At a dose of 2.5 and 5 mg/kg corvitin increased bile content of Chol, PLs and FFAs,and at a dose of 10 mg/kg had the largest effect on the total production of EChol and TGs. We concluded that corvitin activates the liver lipids metabolism and processes of bile formation.
Hydrogen peroxide as a signal mediator at inducing heat resistance in wheat seedlings by putrescine
Yu. E. Kolupaev1,2, A. I. Kokorev1, T. O. Yastreb1, E. I. Horielova1
1Dokuchaev Kharkiv National Agrarian University, Ukraine,
e-mail: plant_biology@ukr.net;
2Karazin Kharkiv National University, Ukraine
Received: 27 May 2019; Accepted: 18 October 2019
Polyamines are multifunctional stress metabolites of plants. However, information on the effect of exogenous polyamines on plant resistance to high temperatures is contradictory, and it remains unclear which signal mediators are involved in the realization of their physiological effects. The possible involvement of hydrogen peroxide as a mediator under the action of exogenous diamine putrescine on the resistance of etiolated wheat seedlings (Triticum aestivum L.) to hyperthermia (10-minute heating at 46°C) and the functioning of antioxidant system was investigated. It was established that the treatment of seedlings with putrescine in 0.25–2.5 mM concentrations caused a significant increase in their heat resistance. In response to the putrescine effect, a transient increase in the H2O2 content occurred in the root cells. This effect was eliminated by treatment of seedlings with a diamine oxidase inhibitor aminoguanidine and an NADPH oxidase inhibitor imidazole. These inhibitors, as well as the scavenger of hydrogen peroxide dimethylthiourea (DMTU), mitigated the effects of increased heat resistance of seedlings and increased activity of superoxide dismutase and catalase caused by putrescine. Under the influence of DMTU and imidazole, but not aminoguanidine, the effect of increasing the activity of guaiacol peroxidase in the roots of seedlings treated with putrescine was eliminated. The conclusion was made about the role of hydrogen peroxide and the possible participation of diamine oxidase and NADPH oxidase in its formation during the implementation of the stress-protective effect of putrescine on wheat seedlings.
Fatty acids composition of Bacillus subtilis ONU551 lipids
Т. V. Gudzenko, O. V. Voliuvach, O. G. Gorshkova,
А. М. Ostapchuk, V. O. Ivanytsia
Оdesa National I.I. Mechnykov University, Ukraine;
e-mail: tgudzenko@ukr.net
Received: 11 March 2019; Accepted: 18 October 2019
The aim of the study was to determine the cellular lipids fatty acid composition for identification of the Bacillus subtilis ONU551 strain bacteria, which is a phenol destructor. Fatty acids analysis of B. subtilis ONU551 strain was performed using an automatic system for microorganisms’ identification MIDI Sherlock (MIDI, USA) based on gas chromatograph Agilent 7890. Chromatograms analysis showed that the fatty acid spectrum of the strain B. subtilis ONU551 consisted predominately of branched structural isomers of saturated acids: 13-methyltetradecanoic (15:0 iso; 34.72%) and 12-methyltetradecanoic (15:0 anteiso; 33.72%) acids. The total content of the branched saturated fatty acids was 88.16% – 14:0 iso (0.52%), 15:0 iso (34.72%), 15:0 anteiso (33.72%), 16:0 iso (1.85%), 17:0 iso (7.11%), 17:0 anteiso (10.24%). The saturated fatty acids of the normal structure were also detected – 12:0 (0.36%), 14:0 (0.28%), 16:0 (1.30%). No 2- and 3-hydroxy acids and no cyclic fatty acids were detected in the fatty acid profile of B. subtilis ONU551 strain. Unsaturated fatty acid isomers – 15:1 w5c (1.85%), 16:1 w11c (1.21%), 16:1 w7c alcohol (1.08%), 17:1 iso w10c (3.18%), ∑17:1 iso I/anteiso B (2.57%) were shown to be the distinctive biomarkers of the B. subtilis ONU551 strain. According to the fatty acid profile analysis with MIDI Sherlock system, the studied strain was identified as Bacillus subtilis with high level of similarity index (0.563).
Effect of selenium and nano-selenium on cisplatin-induced nephrotoxicity in albino rats
M. M. A. Shafaee1, H. S. Mohamed2, S. A. Ahmed1, M. A. Kandeil3
1Chemistry department, Faculty of Science, Beni-Suef University, Egypt;
2Research Institute of Medicinal and Aromatic Plants, Beni-Suef University, Egypt;
3Biochemistry department, Faculty of Veterinary medicine, Beni-Suef University, Egypt;
e-mail: husseinshaban@science.bsu.edu.eg
Received: 05 July 2019; Accepted: 18 October 2019
Cisplatin is commonly used as a chemotherapeutic agent useful in the treatment of several forms of cancer, but its use is limited due to the undesirable side effects of nephrotoxicity. Most of the previous researches found a positive effect of using selenium as an antioxidant on the toxicity of cisplatin during short term administrations although the recommended dose regimen of cisplatin in chemotherapy is multiple successive administration every three or four weeks depending on the type of the tumor. The aim of this study was to examine the effects of long term usage of selenium or nano-selenium on cisplatin-induced nephrotoxicity in albino rats. Forty rats were divided into equal four groups, 1st group as a control injected with normal saline, 2nd group injected with cisplatin 6 mg/kg every 21 days for 70 days (experimental period), 3rd group injected with cisplatin 6 mg/kg plus intramuscular injection 0.1 mg/kg selenium in the form of sodium selenite every 3 days during the experimental period, the 4th group injected with cisplatin 6 mg/kg plus intramuscular injection 0.1 mg/kg nano-selenium every 3 days during the experimental period. The results indicated that selenium or nano-selenium exerted an antioxidant effect through increasing the level of antioxidant enzymes in both serum and kidney tissue, while, it shows a negative effect on kidney function through increasing serum urea and creatinine concentrations and causing abnormal morphology of kidney tissue for rats treated with cisplatin during experimental period.
The concentration of ceruloplasmin in blood of tumor-bearing rats after administration of a dirhenium(III) compound and cisplatin
O. I. Hrabovska1, K. L. Shamelashvili2, O. V. Shtemenko4, N. I. Shtemenko3,4
1Institute of Gastroenterology of the National Academy of Medical Sciences of Ukraine, Dnipro;
2SI “Dnipropetrovsk Medical Academy of the Ministry of Health of Ukraine”, Dnipro;
3Dnipro University of Technology, Ukraine;
4Ukrainian State University of Chemical Technology, Dnipro;
e-mail: n.shtemenko@i.ua
Received: 18 January 2019; Accepted: 18 October 2019
Contemporary investigations of mechanisms of resistance to platinides showed the key role of copper metabolism in cancer patients and proposed possible methods to attenuate the resistance by modulation of copper transporting mechanisms. In this vein, investigation of ceruloplasmin (Cp) levels – the main copper-containing protein in blood, in experiments with tumor-containing animals upon cytostatics administration is topical and has great importance. The concentration of Cp was measured in the serum of tumor-bearing rats with ordinary (T8) and resistant to cisplatin (T8*) Guerin’s carcinoma upon administration of cisplatin and quadruple bonding dirhenium(III) compound dichlorotetra-μ-isobutyratodirhenium(ІІІ) (I) in different medicamental forms. It was shown that development of tumor in T8 group led to increasing of concentration of Cp in 3.7 times and in T8* group – more than in 8 times in comparison to control, confirming the essential role of Cp in the formation of resistance phenomenon. Administration of cisplatin together with I led to effective inhibition of tumor in groups with T8 and T8*, indicating decreased resistance in the group T8*. Greater reduction of Cp levels was observed in the groups with T8* upon administration of the rhenium-platinum antitumor system, than in groups with T8, that underlines the importance of further investigations of the dirhenium(III) compounds in the resistance to cytostatics cancer models. Some mechanisms concerning the regulation of copper homeostasis and properties of nano-composites are discussed.
Oxidative stress and thiols depletion impair tibia fracture healing in young men with type 2 diabetes
H. I. Falfushynska1, O. I. Horyn1, D. V. Poznansky1, D. V. Osadchuk2,
T. О. Savchyn3, T. І. Krytskyi2, L. S. Merva1, S. Z. Hrabra1
1Ternopil Volodymyr Hnatiuk National Pedagogical University, Ukraine;
2I. Horbachevsky Ternopil National Medical University, Ukraine;
3Ternopil Ivan Puluj National Technical University, Ukraine;
e-mail: falfushynska@tnpu.edu.ua
Received: 05 May 2019; Accepted: 18 October 2019
Diabetes mellitus is a metabolic disorder that enhances fracture risk and hinders bone formation. The aim of the present study was to evaluate the parameters of oxidative stress, metallothioneins (MTs), metabolic changes and cytotoxicity signs in blood of young men with (DTF group) and without (TF group) type 2 diabetes (T2D) mellitus who had a tibia fracture due to trauma in relation to specific markers of bone formation. The level of reactive oxygen species was determined using a ROS-sensitive fluorescent dye dihydrorhodamine, DNA fragmentation was detected with Hoescht 33342 fluorescent dye and caspase-3 was assessed in terms of acetyl-Asp-Glu-Val-Asp p-nitroanilide. All other studied indices were determined by standard spectrometric methods. Our results revealed the significant effect of T2D on the bone healing. Indeed, the indices variation in the DTF group were significantly deeper as compared to group TF. The bone fracture in both TF and DTF groups had led to a significant decrease in antioxidants activity and/or level and a consistent increase in signs of oxidative damage. The concentration of MTs was also altered by trauma, but ina group-specific manner: an increase was noted in TF patients after trauma while in diabetes group a decrease in MTs was observed. Likewise, glutathione was strongly suppressed (by -64%) in DTF group. Tibia fracture provoked cytotoxicity which was manifested by increasing lactate dehydrogenase (LDH), cholinesterase and caspase-3 activity, the key effector of apoptosis in osteoclasts. The activity of alkaline phosphatase and total calcium increased only in TF group which demonstrated adequate remodelling process. The most prominent indices for groups splitting include ROS concentration, caspase 3, glutathione transferase and LDH activities mostly conjoint to DTF group. In sum, T2D impairs bone healing under condition of severe oxidative stress and cellular thiols depletion which result in an increase in apoptosis and DNA fragmentation. Our findings establish a biochemical link between increased oxidative stress and reduced bone markers and provide a rational for further studies investigating the role of pro- and antioxidants in bone healing.