Tag Archives: antimicrobial activity

Honeybee chitosan-melanin complex: isolation and investigation of antimicrobial activity

M. Lootsik1, N. Manko1, O. Gromyko2,
S. Tistechok2, M. Lutsyk (Jr.)3, R. Stoika1*

1Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv;
2Ivan Franko National University of Lviv, Ukraine;
3Danylo Halytsky Lviv National Medical University, Ukraine;
*e-mail: stoika.rostyslav@gmail.com

Received: 4 May 2020; Accepted: 13 November 2020

Antimicrobial activity of marine crustaceans chitosans is well studied and is widely used in medicine, while chitosans of insects are poorly investigated in this aspect, though they might also be of practical significance. The aim of this study was to isolate and purify chitosan-melanin complex (CMC) from the honeybee­ corpses and to estimate its antimicrobial activity. Antibacterial activity of CMC was evaluated by MTT test, antifungal activity towards Candida albicans was estimated by calculating colony forming units (CFU method). The modified method of CMC isolation and purification was described which differs from the known analogs in deacetylation of chitin-melanin complex by its hydrolysis in 40% NaOH without previous melanin elimination and in further purification of CMC by differential solubilization at distinct pH values. The anti-microbial activity of CMC was characterized by prevalence of candidacidal effect, IC50 towards laboratory strain of C. albicans was 50 μg/ml. The ranking of studied bacteria sensitivity to the CMC action decreased as: E. coli > St. aureus > Ps. aeruginosa. It is suggested that CMC isolated from the honeybee corpses might be a perspective constituent of medicinal compositions for treatment of lesions caused by C. albicans infection.

Development of the method for microbiological purity testing of recombinant human interleukin-7-based product

O. Yu. Galkin1, T. M. Lutsenko1,2, Yu. V. Gorshunov1, V. V. Motronenko1

1National Technical University of Ukraine
“Ihor Sikorskii Kyiv Polytechnic Institute”, Ukraine;
2UA “PRO-PHARMA” LLC, Kyiv, Ukraine;
e-mail: alexfbt@gmail.com

The goal of the work was justification of standardization parameters of the product based on recombinant human IL-7 by “microbiological purity” parameter and development of its measurement method with consideration of the requirements of guideline documents regarding quality of medicinal pro­ducts. Suitabi­lity assessment of microbiological purity testing procedures was performed. It has been established that the studied­ product in standard testing methods possessed antimicrobial activity against test microorganisms Basillus subtilis ATCC 6633 (on soybean casein digest agar and Sabouraud dextrose agar containing an antibiotic), Candida albicans АТСС 10231 (on soybean casein digest agar and Sabouraud dextrose agar containing an antibiotic), and possessed no antimicrobial activity against test microorganisms Staphylococcus aureus ATCC 6538 (on soybean casein digest agar) and Pseudomonas aeruginosa ATCC 9027 (on soybean casein digest agar). The efficacy of lecithin and polysorbate as substances eliminating antibacterial effect in combination with 50-fold dilution of the test sample has been suggested and proven for the purpose of antimicrobial activity neutralization. The developed total aerobic microbial count measurement procedure for the test product conforms to acceptance criteria provided by SPU (2.6.12), and can be used for its microbiological purity control.

QSAR studies and antimicrobial potential of 1,3-thiazolylphosphonium salts

M. M. Trush1*, V. V. Kovalishyn1, V. M. Blagodatnyi2, V. S. Brovarets1,
S. G. Pilyo1, V. M. Prokopenko1, D. M. Hodyna1, L. O. Metelytsia1

1Institute of Bioorganic Chemistry and Petrochemistry, National Academy
of Sciences of Ukraine, Kyiv;
2Shupyk National Medical Academy of Postgraduate Education, Kyiv, Ukraine;
*e-mail: maria@bpci.kiev.ua

The regression QSAR models were built to predict the antimicrobial activity of new thiazole derivatives. Compounds with high predicting activity were synthesized and evaluated against Gram-positive and Gram-negative bacteria and fungi. 1,3-Thiazole-4-ylphosphonium salts 4 and 5 displayed good antibacterial properties and high antifungal activity. The predictions are in a good agreement with the experiment results, which indicate the good predictive power of the created QSAR models.