Ukr.Biochem.J. 2014; Volume 86, Issue 4, Jul-Aug, pp. 126-131


State of antioxidant system of rat thymocytes in experimental ulcerogenesis

V. A. Kovaleva, L. M. Gaida, A. E. Shevchenko, D. V. Shelest, L. I. Ostapchenko

Educational and Scientific Centre Institute of Biology,
Taras Shevchenko Kyiv National University, Ukraine;

Features of free radical processes and their impact on the implementation of immunocompetent cells of their functions under conditions of peptic ulcer are insufficiently studied today. Reduced activity of catalase 1.7 and 3.4 times and that of glutathione peroxidise  ~ 2.0 times, accordingly, were observed in both models of gastric ulceration (stress ulcer and ethanol one). Enzymatic activity of superoxide dismutase decreased 1.5 times and activity of glutathione transferase increased 1.8 times in the stress model in contrast to the ethanol model of stomach ulcer. Obtained results indicate the exhaustion of antioxidant system in rats’ thymocytes under experimental ulcerogenesis. These data confirm complex negative effect of ulcer on the organism.

Keywords: , , , , ,


  1. Saenko VF, Gomolyako IV, Buryi AN. Peculiar properties of associated with gelicobakteriosis stomach and duodenum diseases’ diagnostics and treatment in the surgical clinic. Klіnіchna Hіrurgіya. 2001;(6):14-18. (In Ukrainian).
  2. Freydlyn IS. Immune System and its defects. S-Peterbyrg, 1998. P. 8-9. (In Russian).
  3. Zapadnyuk IP, Zapadnyuk VI, Zacharya ЕА, Zapadnyuk BV. Laboratory animals. Breeding, maintenance, use in experiment. K.: Vishcha shkola, 1983. 383 p. (In Russian).
  4. Practitioner’s guide of gastroenterology. Eds. by VT Ivashkin, SI Rapoport. М.: Sovetskiy Sport, 1999. 432 р. (In Russian).
  5. Groisman SD, Karevina TG. The effect of atropine on the stress damages of rats’ gastric mucosal. Bibl. Ukaz. VINITI. Dep. Rukopisi. 1979;131(12). (In Ukrainian).
  6. Borysov SI, Hrynyuk II, Kovalova VA, Ptytsya OM, Matyshevs’ka OP. Obtaining and characterization of drug cores lymphocytes of the thymus and spleen of rats. Bull Kyiv Taras Shevchenko Univ. Biology. 1999;29:12–14. (In Ukrainian).
  7. Csóvári S, Andyal T, Strenger J. Determination of the antioxidant properties of the blood and their diagnostic significance in the elderly. Lab Delo. 1991;(10):9-13. Russian. PubMed
  8. Koroliuk MA, Ivanova LI, Mayorova IG, Tokarev VE. A method of determining catalase activity. Lab Delo. 1988;(1):16-9. Russian. PubMed
  9. Gerush ІV, Meschishen IV. State of blood glutatіon system under experimental ulcer damage of gastroduodenal zone and effect of ehіnatseya purpurova’s tincture. Vіsn. Problem Bіol Med. 1998;(7):10–15. (In Ukrainian).
  10. Meschishen I. F. Method of determination glutathione’s activity in blood. In.: Application of enzymes in medicine. Simferopol, 1987. 35 p. (In Russian).
  11. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265-75.
  12. Baraboy VA, Sutkovoy DA. Ed. Zozulуa UA. Oxidation-antioxidant homeostasis under norma and pathology. Kyiv: Naukova dumka, 1997. 357 p. (In Russian).
  13. Li Y, Huang TT, Carlson EJ, Melov S, Ursell PC, Olson JL, Noble LJ, Yoshimura MP, Berger C, Chan PH, Wallace DC, Epstein CJ. Dilated cardiomyopathy and neonatal lethality in mutant mice lacking manganese superoxide dismutase. Nat Genet. 1995 Dec;11(4):376-81. PubMed, CrossRef
  14. Halliwell B, Gutteridge JMC. Lipid peroxidation, oxygen radicals, cell damage, and antioxidant therapy. Lancet. 1984 Jun 23;323(8391):1396-7. PubMed, CrossRef
  15. Hotchkiss RS, Osmon SB, Chang KC, Wagner TH, Coopersmith CM, Karl IE. Accelerated lymphocyte death in sepsis occurs by both the death receptor and mitochondrial pathways. J Immunol. 2005 Apr 15;174(8):5110-8. PubMed, CrossRef

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License.