Ukr.Biochem.J. 2021; Volume 93, Issue 1, Jan-Feb, pp. 75-81

doi: https://doi.org/10.15407/ubj93.01.075

Organo-specific accumulation of phenolic compounds in a buckwheat seedlings under aluminium-acid stress

O. E. Smirnov, A. M. Kosyan, Yu. V. Pryimak,
O. I. Kosyk, N. Yu. Taran

ESC “Institute of Biology and Medicine”, Taras Shevchenko National University of Kyiv, Ukraine;
e-mail: plantaphys@gmail.com

Received: 19 September 2020; Accepted: 17 December 2020

Toxic effect of aluminum contamination is one of the causes of valuable crops yield loss all over the world. It is considered that plants’ phenolic compounds play a key role in aluminium detoxification by chelation of aluminium ions in the aboveground part of aluminium-accumulating plants. However, recent evidence shows the chelating ligands involvement in both the internal and external aluminium detoxification in plants. The aim of the study was to determine the total phenolic compounds, flavonoids, anthocyanins accumulation and the activity of phenylalanine ammonia-lyase (PAL) as the key enzyme in phenolic compounds synthesis in seedlings of common (Fagopyrum esculentum Moench.) and tartary (Fagopyrum tataricum (L.) Gaertn.) buckwheat in response to the chronic aluminium-acid stress. It was recorded that addition of 50 μM Al2(SO4)3·18H2O to the nutrient medium led to the accumulation of phenolic compounds in all organs of both studied species on the tenth day of the plant exposure to stress. Species-specific and organ-specific accumulation of certain classes of phenylpropanoids was recorded. On the tenth day of stress, PAL activity was increased in the leaf tissues of both buckwheat species, but was decreased in common buckwheat root tissues and no statistically significant changes were observed in tartary buckwheat root tissues. Species and organ specificity of phenylpropanoids accumulation in the studied species is considered to be an adaptive reaction under conditions of aluminum stress.

Keywords: , , , ,


References:

  1. Zhu JK. Abiotic Stress Signaling and Responses in Plants. Cell. 2016;167(2):313-324. PubMed, PubMedCentral, CrossRef
  2. Van Emon JM. The Omics Revolution in Agricultural Research. J Agric Food Chem. 2016;64(1):36-44. PubMed, PubMedCentral, CrossRef
  3.  Krishnamurthy A, Rathinasabapathi B. Oxidative stress tolerance in plants: novel interplay between auxin and reactive oxygen species signaling. Plant Signal Behav. 2013;8(10):e25761. PubMed, PubMedCentral, CrossRef
  4. Vighi IL, Benitez LC, Amaral MN, Moraes GP, Auler PA, Rodrigues GS, Deuner S, Maia LC, Braga EJB. Functional characterization of the antioxidant enzymes in rice plants exposed to salinity stress. Biologia Plantarum. 2017;61(3):540-550. CrossRef
  5. Nahak G, Suar M, Sahu RH. Antioxidant Potential and Nutritional Values of Vegetables: A Review. Res J Med Plants. 2014;8(2):50-81. CrossRef
  6. Babenko LM, Smirnov OE, Romanenko KO, Trunova OK, Kosakivska IV. Phenolic compounds in plants: biogenesis and functions. Ukr Biochem J. 2019;91(3):5-18. CrossRef
  7. Rahman R, Upadhyaya H. Aluminium Toxicity and Its Tolerance in Plant: A Review. J Plant Biol. 2020;63(5). CrossRef
  8. Yang ZB, Rao IM, Horst WJ. Interaction of aluminium and drought stress on root growth and crop yield on acid soils. Plant Soil. 2013;372(1-2):3-25. CrossRef
  9. Zhang X, Long Y, Huang J, Xia J. Molecular Mechanisms for Coping with Al Toxicity in Plants. Int J Mol Sci. 2019;20(7):1551. PubMed, PubMedCentral, CrossRef
  10. Ma JF. Role of organic acids in detoxification of aluminum in higher plants. Plant Cell Physiol. 2000;41(4):383-390. PubMed, CrossRef
  11. Bobo-García G, Davidov-Pardo G, Arroqui C, Vírseda P, Marín-Arroyo MR, Navarro M. Intra-laboratory validation of microplate methods for total phenolic content and antioxidant activity on polyphenolic extracts, and comparison with conventional spectrophotometric methods. J Sci Food Agric. 2015;95(1):204-209. PubMed, CrossRef
  12. Babenko LM, Vodka MV, Akimov YuN, Smirnov AE, Babenko AV, Kosakovskaya IV. Specific features of the ultrastructure and biochemical composition of Triticum spelta L. leaf mesophile cells in the initial period of stress temperature action. Cell Tissue Biol. 2019;13(1):70-78. CrossRef
  13. Li X, Kim JK, Park SY, Zhao S, Kim YB, Lee S, Park SU. Comparative analysis of flavonoids and polar metabolite profiling of Tanno-original and Tanno-high rutin buckwheat. J Agric Food Chem. 2014;62(12):2701-2708. PubMedCrossRef
  14. Jaleel CA, Wang G, Ahmad P. Changes in the photosynthetic characteristics of Catharanthus roseus L. as a result of exogenous growth regulators. Plant Omics J. 2009;2(4):169-174.
  15. Zucker M. Induction of Phenylalanine Deaminase by Light and its Relation to Chlorogenic Acid Synthesis in Potato Tuber Tissue. Plant Physiol. 1965;40(5):779-784. PubMed, PubMedCentral, CrossRef
  16. Smirnov OE, Kosyan AM, Kosyk OI, Taran  NYu. Response of phenolic metabolism induced by aluminium toxicity in Fagopyrum esculentum Moench. plants. Ukr Biochem J. 2015;87(6):129-135.  PubMed, CrossRef
  17. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72(1-2):248-254. PubMed, CrossRef
  18. Procházková D, Boušová I, Wilhelmová N.  Antioxidant and prooxidant properties of flavonoids. Fitoterapia. 2011;82(4):513-523. PubMed, CrossRef
  19. Kosyk OI, Khomenko IM, Batsmanova LM, Taran NYu. Phenylalanine ammonia-lyase activity and anthocyanin content in different varieties of lettuce under the cadmium influence. Ukr Biochem J. 2017;89(2):85-91. CrossRef
  20. Jaskowiak J, Tkaczyk O, Slota M, Kwasniewska J, Szarejko I. Analysis of aluminum toxicity in Hordeum vulgare roots with an emphasis on DNA integrity and cell cycle. PLoS One. 2018;13(2):e0193156.PubMed, PubMedCentral, CrossRef
  21. Liu Q, Yang JL, He LS, Li YY, Zheng SJ. Effect of aluminum on cell wall, plasma membrane, antioxidants and root elongation in triticale. Biologia Plantarum. 2008;52(1):87-92.  CrossRef
  22. Zhu CQ, Hu WJ, Cao XC, Zhu LF, Bai ZG, Huang J, Liang QD, Jin QYu, Zhang JH. Role of salicylic acid in alleviating the inhibition of root elongation by suppressing ethylene emission in rice under Al toxicity conditions. Plant Growth Regul. 2020;90(3):475-487.
  23. Wang H, Chen RF, Iwashita T, Shen RF, Ma JF.Physiological characterization of aluminum tolerance and accumulation in tartary and wild buckwheat. New Phytol. 2015;205(1):273-279. PubMed, CrossRef

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