Tag Archives: phenolic compounds

Organo-specific accumulation of phenolic compounds in a buckwheat seedlings under aluminium-acid stress

O. E. Smirnov, A. M. Kosyan, Yu. V. Pryimak,
O. I. Kosyk, N. Yu. Taran

ESC “Institute of Biology and Medicine”, Taras Shevchenko National University of Kyiv, Ukraine;
e-mail: plantaphys@gmail.com

Received: 19 September 2020; Accepted: 17 December 2020

Toxic effect of aluminum contamination is one of the causes of valuable crops yield loss all over the world. It is considered that plants’ phenolic compounds play a key role in aluminium detoxification by chelation of aluminium ions in the aboveground part of aluminium-accumulating plants. However, recent evidence shows the chelating ligands involvement in both the internal and external aluminium detoxification in plants. The aim of the study was to determine the total phenolic compounds, flavonoids, anthocyanins accumulation and the activity of phenylalanine ammonia-lyase (PAL) as the key enzyme in phenolic compounds synthesis in seedlings of common (Fagopyrum esculentum Moench.) and tartary (Fagopyrum tataricum (L.) Gaertn.) buckwheat in response to the chronic aluminium-acid stress. It was recorded that addition of 50 μM Al2(SO4)3·18H2O to the nutrient medium led to the accumulation of phenolic compounds in all organs of both studied species on the tenth day of the plant exposure to stress. Species-specific and organ-specific accumulation of certain classes of phenylpropanoids was recorded. On the tenth day of stress, PAL activity was increased in the leaf tissues of both buckwheat species, but was decreased in common buckwheat root tissues and no statistically significant changes were observed in tartary buckwheat root tissues. Species and organ specificity of phenylpropanoids accumulation in the studied species is considered to be an adaptive reaction under conditions of aluminum stress.

Phenolic compounds in plants: biogenesis and functions

L. M. Babenko1, O. E. Smirnov2, K. O. Romanenko1,
O. K. Trunova3, I. V. Kosakіvskа1

1M.G. Kholodny Institute of Botany, National Academy of Sciences of Ukraine, Kyiv;
2Educational and Scientific Center “Institute of Biology and Medicine”, Taras Shevchenko National University of Kyiv, Ukraine;
3V.I. Vernadsky Institute of General and Inorganic Chemistry, National Academy of Sciences of Ukraine, Kyiv;
e-mail: lilia.babenko@gmail.com

Received: 05 November 2018; Accepted: 14 March 2019

Phenolic compounds (PCs) in plants play an important role in growth control and have antioxidant, structural, attractant, signaling and protective functions. Information on the discovery, study and identification of phenolic compounds in plants, their role in the complex system of secondary metabolites has been analyzed and summarized. The functions of PCs at the macromolecular, cellular as well as organism and population levels are described. The pathways of PCs formation, enzymes responsible for their synthesis and the plasticity of the synthesis in a plant cell are highlighted. The involvement of PCs in the plant breathing, photosynthesis, oxidation-reduction processes and regulation of the plants physiological state are discussed.

Response of phenolic metabolism induced by aluminium toxicity in Fagopyrum esculentum Moench. plants

O. E. Smirnov, A. M. Kosyan, O. I. Kosyk, N. Yu. Taran

Educational and Scientific Centre “Institute of Biology”,
Taras Shevchenko National University of Kyiv, Ukraine;
e-mail: plantaphys@gmail.com

Buckwheat genus (Fagopyrum Mill.) is one of the aluminium tolerant taxonomic units of plants. The aim of the study was an evaluation of the aluminium (50 µM) effect on phenolic accumulation in various parts of buckwheat plants (Fagopyrum esculentum Moench.). Detection of increasing of total phenolic content, changes in flavonoid and anthocyanin content and phenylalanine ammonia-lyase activity (PAL) were revealed over a period of 10 days of exposure to aluminium. The most significant effects of aluminium treatment on phenolic compounds accumulation were total phenolic content increasing (by 27.2%) and PAL activity rising by 2.5 times observed in leaves tissues. Received data could be helpful to understand the aluminium tolerance principles and relationships of phenolic compounds to aluminium phytotoxicity.