Ukr.Biochem.J. 2021; Volume 93, Issue 1, Jan-Feb, pp. 96-103

doi: https://doi.org/10.15407/ubj93.01.096

Quercetin and histamine effects on free radical reactions in rat erythrocytes

15.02.2021   Uncategorized   No comments

N. P. Harasym*, M. Y. Booklyv, A. R. Zyn, S. M. Mandzynets,
A. O. Bezkorovainy, D. I. Sanahursky

Ivan Franko National University of Lviv, Faculty of Biology, Departments of Biophysics and Bioinformatics, Ukraine;
*e-mail: garasymnataly@gmail.com

Received: 12 June 2020; Accepted: 17 December 2020

The effects of quercetin and histamine separately or in combination on the free radical state of rat erythrocytes were estimated in vitro. Quercetin (0.1; 0.5; 3.0; 5.0 mM) or histamine (0.01; 10.0 μM) were added to whole blood separately or in combination. The content of hydroperoxides, TBA-active products and carbonyl groups of proteins in erythrocytes after hemolysis was determined. The greatest influence of quercetin and histamine on erythrocytes state indicators was revealed under their combined action, when the level of TBA-active products and the content of carbonyl groups of proteins were found to be increased substantially.

Keywords: , , , ,

References:

  1. Demkovych A. Effects of flavonol quercetin on activity of lipid peroxide oxidation in experimental bacterial-immune periodontitis. Interv Med Appl Sci. 2019;11(1):55-59. PubMed, PubMedCentral, CrossRef
  2. Niki E. Oxidant-specific biomarkers of oxidative stress. Association with atherosclerosis and implication for antioxidant effects. Free Radic Biol Med. 2018;120:425-440. PubMed, CrossRef
  3. Buddi R, Lin B, Atilan SR, Zorapapel NC, Kenney MC, Brown DJ. Evidence of oxidative stress in human corneal diseases. J Histochem Cytochem. 2002;50(3):341-351. PubMed, CrossRef
  4. Marunaka Y, Marunaka R, Sun H, Yamamoto T, Kanamura N,  Inui T, Taruno A. Actions of Quercetin, a Polyphenol, on Blood Pressure. Molecules. 2017;22(2):209. PubMed, PubMedCentral, CrossRef
  5. Suganthy N, Devi KP, Nabavi SF, Braidy N, Nabavi SM. Bioactive effects of quercetin in the central nervous system: Focusing on the mechanisms of actions. Biomed Pharmacother. 2016;84:892-908.  PubMed, CrossRef
  6. Andres S, Pevny S, Ziegenhagen R, Bakhiya N, Schäfer B, Hirsch-Ernst KI, Lampen A. Safety Aspects of the Use of Quercetin as a Dietary Supplement. Mol Nutr Food Res. 2018;62(1):1700447.  PubMed, CrossRef
  7. Duranti G, Ceci  R, Patrizio F, Sgrò P, Di Luigi L, Sabatini S, Felici F, Bazzucchi I. Chronic consumption of quercetin reduces erythrocytes oxidative damage: Evaluation at resting and after eccentric exercise in humans. Nutr Res. 2018;50:73-81. PubMed, CrossRef
  8. Lieberman P. The basics of histamine biology. Ann Allergy Asthma Immunol. 2011;106(2 Suppl):S2-S5. PubMed, CrossRef
  9. Gurgen SG, Erdogan D, Take-Kaplanoglu G. The effect of histamine on kidney by fasting in rats. Bratisl Lek Listy. 2013;114(5):251-257. PubMed, CrossRef
  10. Chumakova SP, Urazova OI, Zyma AP, Novitskiy VV. Features of the physiology of erythrocytes. Hemolysis and eryptosis. Russ J Hematol Transfusiol. 2018;63(4):343–351. (In Russian). CrossRef
  11. Zervas E, Loukides S, Papatheodorou G, Psathakis K, Tsindiris K, Panagou P, Kalogeropoulos N. Magnesium levels in plasma and erythrocytes before and after histamine challenge. Eur Respir J. 2000;16(4):621-625.  PubMed, CrossRef
  12. Helms CC, Gladwin MT, Kim-Shapiro DB. Erythrocytes and Vascular Function: Oxygen and Nitric Oxide. Front Physiol. 2018;9:125. PubMed, PubMedCentral, CrossRef
  13. Oleksiuk NP, Yanovych VG. The activity of pro- and antioxidant systems in the liver of freshwater fishes during different seasons. Ukr Biokhim Zhurn. 2010;82(3):41-48.  (In Ukrainian). PubMed
  14. Timirbulatov RA, Seleznev EI. Method for increasing the intensity of free radical oxidation of lipid-containing components of the blood and its diagnostic significance. Lab Delo. 1981;(4):209-211. (In Russian). PubMed
  15.  Meschyshyn IF, Poloviy VP Mechanism of oxidative modification of proteins. Buk Med. Herald J. 1999;1:196–205. (In Ukrainian).
  16.  Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951;193(1):265-275. PubMed
  17. Rogovsky VS, Matyushin AI, Shimanovsky NL. The prospects of Quercetinum and its derivatives administration for prevention and treatment of atherosclerosis.  Int Med J. 2011;(3):114–118. (In Russian).
  18. Golovchak NP, Tarnovskaya AV, Kotsyumbas GI, Sanagursky DI Processes of lipid peroxidation in living organisms. Lviv: Ivan Franko National University of Lviv, 2012. 250 p. (In Ukrainian).
  19. Ivasivka RS, Myronenko II. Influence of the quercetine on the dynamics IL-4, general IgE and cortisol as markers of the inflammation respiratory tract activity at bronchial asthma. Exp Clin Physiol Biochem. 2011;(2):99-103. (In Ukrainian).
  20. Fadeyeva HA. The role of mediators of inflammation and atopy in burdening of bronchial asthma course, associated with visceral obesity. Visnyk Sumy State Univy. Med Ser. 2009; 1(2): 162-167. (In Ukrainian).
Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License.