M. A. Shkliarevskyi¹, Yu. E. Kolupaev^1,2*, T. O. Yastreb¹,
Yu. V. Karpets¹, A. P. Dmitriev³

¹Dokuchaev Kharkiv National Agrarian University, Ukraine;
²Karazin Kharkiv National University, Ukraine;
³Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, Kyiv;
*e-mail: plant.biology.knau@gmail.com

Received: 6 December 2020; Accepted: 17 May 2021

The role of the gasotransmitter carbon monoxide (CO) in signaling and adaptive processes in plants has been studied insufficiently. There are indirect data indicating jasmonate signaling participation in realization of CO effects, but  the possible connection between carbon monoxide and jasmonate signaling during plant adaptation to salt stress remains unclear. We studied the carbon monoxide donor hemin effect on the Arabidopsis of a wild-type (Col-0) and defective in jasmonate signaling coi1 and jin1 mutants response to the salt stress.  Arabidopsis thaliana 4-week-old plants were grown on a modified Hoagland’s medium. Plants were incubated for 24 h in usual or 2 µM hemin containing culture medium, then transferred to 150 mM NaCl containing media and incubated for 24 h before the medium was replaced with the usual one. It was shown that salt stress caused water deficiency and superoxide dismutase and catalase activity decrease in the plants of all three genotypes. Treatment with 2 μM hemin stabilized the levels of catalase activity and  photosynthetic pigments and increased guaiacol peroxidase activity in a wild-type, but not in  coi1 and jin1 mutant plants after  stress induction. Treated with hemin wild-type Arabidopsis plants accumulated more proline and sugars in response to stress than treated coi1 and jin1 mutants. It was concluded that jasmonate signaling can be involved in adaptive processes induced by exogenous carbon monoxide.

Keywords: antioxidant enzymes, carbon monoxide, coi1 and jin1 mutants, jasmonate signaling, pigments, proline, salt resistance, wild type Arabidopsis thaliana