Ukr.Biochem.J. 2021; Volume 93, Issue 3, May-Jun, pp. 61-67

doi: https://doi.org/10.15407/ubj93.03.061

Prooxidant and antioxidant processes in the liver homogenate of healthy and tumor-bearing mice under the action of thiazole derivatives

01.07.2021   Uncategorized   No comments

Ya. R. Shalai1*, M. V. Popovych1, S. M. Mandzynets1,
V. P. Hreniukh1, N. S. Finiuk1,2, A. M. Babsky1

1Ivan Franko National University of Lviv, Ukraine;
2Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv;
*e-mail: Yaryna.Shalay@lnu.edu.ua

Received: 12 October 2021; Accepted: 17 May 2021

Thiazole derivatives were shown to have toxic effects in vitro on cancer cells of different origin and can be considered as potentially antineoplastic, but their effect on the normal tissues needs to be studied. In this research the newly synthesized thiazole derivatives of N-(5-benzyl-1,3-thiazol-2-yl)-3,5-dimethyl-1-benzo-furan-2-carboxamide (BF1) and 8-methyl-2-Me-7-[trifluoromethyl-phenylmethyl]-pyrazolo [4,3-e] [1,3] thiazolo [3,2-a] pyrimidin-4 (2H)-one (PP2) were used and their effect on the pro- and antioxidant processes after adding in a 1, 10 and 50 μM concentrations to the liver homogenate of healthy and NK/Ly lymphoma-bearing mice was estimated. The level of superoxide radical and TBA-active products as well as catalase, SOD and glutathione peroxidase activity were measured. It was shown that superoxide radical and TBA-active products­ level and catalase activity were significantly higher in the liver of tumor-bearing mice than in the liver of the healthy mice. Neither BF1 no PP2 influenced the studied indices in the liver homogenate of healthy and tumor-bearing animals with the exception that PP2 significantly reduced the level of TBA-positive products in both cases. The data obtained showed that the studied thiazole derivatives did not cause severe liver toxicity in both healthy and tumor-bearing mice.

Keywords: , , , ,

References:

  1. Finiuk NS, Hreniuh VP, Ostapiuk YuV, Matiychuk VS, Frolov DA, Obushak MD, Stoika RS, Babsky AM. Antineoplastic activity of novel thiazole derivatives. Biopolym Cell. 2017;33(2):135–146. CrossRef
  2. Finiuk N, Klyuchivska O, Ivasechko I, Hreniukh V, Ostapiuk Yu, Shalai Ya, Panchuk R, Matiychuk V, Obushak M, Stoika R, Babsky A. Proapoptotic effects of novel thiazole derivative on human glioma cells. Anticancer Drugs. 2019;30(1):27-37. PubMed, CrossRef
  3. Shalai YaR, Mandzynets SM, Hreniukh VP, Finiuk NS, Babsky AM.  Free-radical processes in NK/Ly lymphoma cells and hepatocytes under the effect of thiazole derivative. Bull Probl Biol Med. 2018;1.2(143):234-238. (In Ukrainian). CrossRef
  4. Shalai YaR, Popovych MV, Kulachkovskyy OR, Hreniukh VP, Mandzynets SM, Finiuk NS, Babsky AM. Effect of novel 2-amino-5-benzylthiazole derivative on cellular ultrastructure and activity of antioxidant system in lymphoma cells. Studia Biologica. 2019;13(1):51-60. CrossRef
  5. Bazinet L, Doyen A. Antioxidants, mechanisms, and recovery by membrane processes. Crit Rev Food Sci Nutr. 2017;57(4):677-700. PubMed, CrossRef
  6. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951;193(1):265-275. PubMed
  7. Gavrilov VB, Mishkorudnaya MI. Spectrophotometric determination of the content of lipid hydroperoxides in blood plasma. Lab Delo. 1983;(3): 33–35.
  8. Timirbulatov RA, Seleznev EI. Method for increasing the intensity of free radical oxidation of lipid-containing components of the blood and its diagnostic significance. Lab Delo. 1981;(4):209-211. (In Russia). PubMed
  9. Denisenko SV, Kostenko VA. Changes in the production of reactive oxygen species in the testes of white rats under conditions of chronic intoxication with sodium nitrate. Modern Probl Toxicol. 2002;(4):44-46. (In Russian).
  10. Korolyuk МА, Ivanova LI, Mayorova IH, Tokaryev VYe. A method of determining catalase activity. Lab Delo. 1988;(1):16-19. (In Russian). PubMed
  11. Kostyuk VA, Potapovich AI, Kovaleva ZnV. Kostiuk VA, Potapovich AI, Kovaleva ZhV. A simple and sensitive method of determination of superoxide dismutase activity based on the reaction of quercetin oxidation. Vopr Med Khim. 1990;36(2):88-91. (In Russian).  PubMed
  12. 12. Moin VM. A simple and specific method for determining glutathione peroxidase activity in erythrocytes. Lab Delo. 1986;(12):724-727. (In Russian).
    PubMed
  13. Demkiv IY, Lisnychuk NY, Soroka YV, Chykhyra OV. Redox balance in white rats’ spleen under induced carcinogenesis. Med Clin Chem. 2016;(3):38–42. (In Ukrainian). CrossRef
  14. Rahmouni A, Souiei S, Belkacem MA, Romdhane A, Bouajila J, Ben Jannet H. Synthesis and biological evaluation of novel pyrazolopyrimidines derivatives as anticancer and anti-5-lipoxygenase agents. Bioorg Chem. 2016;66:160-168. PubMed, CrossRef
Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License.