Tag Archives: antioxidant system

The liver and kidneys biochemical indices at the experimental pancreatitis in case of the administration of nitric oxide synthesis modulators and recombinant superoxide dismutase

O. Z. Yaremchuk, K. A. Posokhova

SHEI «I. Ya. Horbachevsky Ternopil State Medical University», Ukraine;
e-mail: yaremchukolya@rambler.ru

The rats liver and kidneys function indices were studied in case of administration of recombinant superoxide dismutase drug, precursor of nitric oxide L-arginine and the blocker of inducible NO-synthase aminoguanidine. The disturbances in functioning of prooxidant-antioxidant system (a decrease of activity of superoxide dismutase, katalaze, amount of restored glutathione, growth of the level of hydroperoxide lipids, TBA-active products­), mitochondrial electron-transport pathways (a decrease in activity of succinatedehydrogenaze, cytochrome oxydaze), a rise of nitrite-anion level in the liver and kidneys, increase of α-amylase activity and tumor necrosis factor α serum concentration were established on the model of pancreas injury in white male rats. Under these circumstances aminoguanidine attenuated the oxidative stress in the liver and kidneys due to normalization of nitric oxide synthesis. The ability to activate the antioxidant system was proved by combined usage of recombinant superoxide dismutase and aminoguanidine. It was determined that recombinant superoxide dismutase partially decreases the negative influence of L-arginine and improves the biochemical indices of the liver and kidneys function in rats with acute experimental pancreatitis.

Tissue specificity of lipid peroxidation under emotional stress in rats

K. O. Menabde, G. M. Burjanadze, M. V.  Сhachua,
Z. T. Kuchukashvili, N. I. Koshoridze

Iv. Javakhishvili Tbilisi State University, Georgia;
e-mail: ketimenabde@yahoo.com

The intensity of lipid peroxidation and activi­ty of antioxidant system enzymes in the blood plasma, brain and cardial muscle of laboratory rats under 40 days of isolation and violation of diurnal cycle was studied. The obtained data show that on the background of concentration changes in NO changes also take place in the intensity of lipid peroxidation process, indicated by changes in the concentration of TBA-active products and diene conjugates.
The changes taking place in the activity of superoxidedismutase, catalase, succinatdehydrogenase, creatine kinase and aldolase under stress were studied.
The resulting data show that isolation of animals and violation of diurnal cycle are the factors causing a significant reduction in the energy metabolism in the brain and heart tissue cells and resulting in oxidative stress that, in its turn, may become the reason for development of toxic radicals.
Furthermore, prolonged stress may result in irreversible processes that are considered to be the reasons for significant pathologies of the cardiovascular system.

Antioxidant system activation in rats with experimental cirrhosis after injection of cryopreserved fetal liver cells

О. V. Ochenashko1, Yu. V. Nikitchenko2, А. S. Lebedinsky1,
A. N. Sukach1, A. Yu. Petrenko1

1Institute for Problems of Cryobiology and Cryomedicine,
National Academy of Sciences of Ukraine, Kharkiv;
2Research Institute of Biology, V. N. Karazin Kharkiv
National University, Ukraine;
e-mail: ochenashko@ukr.net

The possibility to recover the antioxidant system in rats with experimental liver cirrhosis (LC) after allo- and xenotransplantation of cryopreserved fetal liver cells (FLC) was investigated. It was shown that the content of lipid peroxidation products in the blood serum of animals with LC four weeks after FLC transplantation decreased significantly as compared to control group. Such changes were accompanied by a significant increase of catalase (CAT), glutathione reductase (GR), Se-dependent glutathione peroxidase (GP) activity in the liver and total anti-oxidative activity (AOA) of blood.
Obtained results demonstrate that the main direction of FLC effects in animals with LC agree with that we observed previously in other experimental models (partial hepatectomy, chronic alcohol poisoning and hypercholesterolemia). In conclusion, cell therapy may be considered as the universal method for correction of disorders in regulation of free-radical processes in various experimental pathologies.

Oxidative stress in blood leukocytes, pro/antioxidanт status and fatty acids composition of pancreas lipids at experimental acute pancreatitis in rats

I. B. Pryvrotska1, T. M. Kuchmerovska2

1I. Нorbachevsky Ternopyl State Medical University, Ukraine;
2Palladin Institute of Biochemistry, National Academy of Science of Ukraine, Kyiv;
e-mail: irina.privrotska@yandex.uа

In an experimental model of acute pancreatitis (АP) in rats no alteration in leukocyte’s viability was found by flow cytometry as compared to control. After 1 day of AP production of reactive oxygen forms in granulocytes was increased more than 5 times, but after 3 days their level was decreased. Alterations of pro/antioxidant status and specific changes in the fatty acid composition in the pancreas were established. With the development of AP, the processes of lipids peroxidation were intensified while antioxidant system was altered, that was evidenced by inflammation in the pancreas. In these conditions, the increase of phospholipase A2 activity was accompanied by significant changes of fatty acid composition of the total lipids in the pancreas. This increased relative total content of saturated fatty acids, in particular myristic, palmitic and stearic acid increased, while the total content of polyunsaturated essential fatty acids ω-3 (linolenic, eicosapentaenoic, dokozapentayenoic, docosahexaenoic) decreased. The preparation containing ω-3 polyunsaturated fatty acids partially normalized the lipid and fatty acids composition as well as prooxidant-antioxidant system.

Peculiarities of antioxidant defense system in erythroid cells and tissues of pigs under action of chromium chloride

R. Ja. Iskra, V. V. Vlizlo

Institute of Animal Biology, National Academy of Agrarian Sciences of Ukraine, Lviv;
e-mail: iskra_r@ukr.net

The influence of CrCl3 in the amount of 400 mg Cr/kg of feed on antioxidant defense in populations of erythrocytes, erythroid bone marrow cells and tissues of pigs was studied. The increasing of the antioxidant defense of swine organism, as evidenced by the increase in superoxide dismutase and glutathione peroxidase activity in the fractions of “young” erythrocytes, was shown. Superoxide dismutase activity decreases, while glutathione and catalase activity increases in the erythroid cells of the bone marrow after of CrCl3 action. Oxidative processes are intensified in the liver of pigs of the experimental group, in contrast to other tissues, leading to the increase of content of TBARS-products, growth of superoxi­de dismutase activity and reduction of glutathione peroxidase activity. At the same time, the action of CrCl3 in other tissues activates antioxidant system, including the kidneys, lungs and myocardium, increases superoxide dismutase activity, and catalase activity in the spleen and kidneys. A decrease of content of TBARS-products and reduction of superoxide dismutase activity, as well as the increase of katalase activity and reduction of glutathione content were discovered in the skeletal muscles of pigs of the experimental group. As a result of research it is suggested to add CrCl3 to the diet of pigs to enhance antioxidant defense during their intensive growth.

Stress-responsive systems in rat pancreas upon long-term gastric hypochlorhydria and administration of multiprobiotic “Symbiter®”

K. O. Dvorshchenko, S. Ye. Vakal, A. S. Dranitsina, S. A. Senin, L. I. Ostapchenko

Taras Shevchenko National University of Kyiv, Ukraine;
e-mail: k21037@gmail.com

The intensity of free-radical processes upon long-term omeprazole-induced hypoacidity in the rat pancreas was investigated. Significant violation of oxidative-antioxidative balance in pancreatic tissue upon gastric hypochlorhydria was established: overproduction of superoxide anion, quantitative changes of lipid functional groups, increased level of lipid peroxidation products, augmentation of xanthine oxidase, superoxide dismutase and glutathione transferase activity, as well as depletion of catalase, glutathione peroxidase activity and reduced glutathione content. The inflected expression of Cckbr gene in the rat pancreas upon these conditions was also observed, thus suggesting an increased risk of pathological changes development in the gland. Abovementioned parameters were only partially restored to control values in the case of simultaneous administration of multiprobiotic “Symbiter®” with omeprazole, thus indicating the ability of this preparation to efficiently counteract the development of oxidative damages in pancrea­tic tissues upon long-term hypoacidic conditions.