Category Archives: Uncategorized
Experimental preeclampsia development depends on vitamin D(3) status in female wistar rats
I. V. Poladych1*, I. O. Shymanskyi2, M. M. Veliky2, D. O. Govsieiev1
1Department of Obstetrics and Gynecology No 1,
Bogomolets National Medical University, Kyiv, Ukraine;
2Department of Biochemistry of Vitamins and Coenzymes,
Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
*e-mail: iren.poladich@gmail.com
Received: 08 May 2025; Revised: 08 August 2025;
Accepted: 12 September 2025; Available on-line: 17 September 2025
Deficiency of vitamin D3 during pregnancy is a widespread challenge associated with increased risk of complications, particularly preeclampsia (PE), a serious condition characterized by hypertension with proteinuria. This research aimed to study the experimental preeclampsia rates in pregnant rats depending on the vitamin D3 supply. Eight-week-old female Wistar rats were divided into three experimental groups: control; vitamin D3-deficient for 60 days before mating; vitamin D3-deficient with oral vitamin D3 supplement (1000 IU/kg b.w.t) two weeks before mating. Subgroups with and without PE induction were analyzed. PE was induced by administration of Nω-nitro-L-arginine methyl ester (L-NAME). The blood level of vitamin D3 was measured using a 25-Hydroxyvitamin D3 ELISA kit. Proteinuria was assessed using semi-quantitative urine test strips “Prototest”. The highest blood pressure and proteinuria levels were recorded in animals with combined vitamin D3 deficiency and induced preeclampsia. Administration of vitamin D3 contributed to normalization of hemodynamic parameters and kidney function, indicating the importance of an adequate vitamin D3 status for pregnancy health and PE prevention.
Chemerin-adiponectin axis in hypothyroidism
M. K. Najim1*, A. F. Al-Shukri2
1Department of Medical Laboratory Technology, College of Health and Medical Technology,
Al-Furat Al-Awsat Technical University, Kufa, Iraq;
Thi-Qar Health Directorate, Al-Rifae Teaching Hospital;
*e-mail: murtadha.najeem.chm@student.atu.edu.iq;
2Department of Medical Laboratory Technology, College of Health and Medical Technology,
Al-Furat Al-Awsat Technical University, Kufa, Iraq;
e-mail: kin.ebt@atu.edu.iq
Received: 09 April 2025; Revised: 30 June 2025;
Accepted: 12 September 2025; Available on-line: 17 September 2025
Hypothyroidism disrupts energy and metabolism due to insufficient thyroid hormones production, leading to metabolic disorders such as insulin resistance and dyslipidemia. Recent studies have demonstrated the impact of adipokines, chemerin and adiponectin on thyroid function. This review analyzes the involvement of these hormones in the metabolic and inflammatory complications of hypothyroidism, their effects and interactions through complex signaling pathways, as well as their possible contribution to the etiology and treatment of hypothyroidism, considering the importance of integrating biomarker data.
Astaxanthin as an antioxidant: exploring its potential in prevention of mitochondrial dysfunction
A. A. Badri1*, N. N. Ayu Dewi2, I. A. I. Wahyuniari3
1Master Program in Biomedical Science, Anti-Aging Medicine,
Faculty of Medicine, Universitas Udayana, Denpasar, Indonesia;
2Department of Biochemistry, Faculty of Medicine, Universitas Udayana, Denpasar, Indonesia;
3Department of Histology, Faculty of Medicine, Universitas Udayana, Denpasar, Indonesia;
*e-mail: ameliabadri940@gmail.com
Received: 03 February 2025; Revised: 21 May 2025;
Accepted: 11 June 2025; Available on-line: 07 July 2025
Astaxanthin is a natural carotenoid with a powerful antioxidant activity, high stability and the ability to cross both the blood-brain and the blood-retinal barriers. It demonstrates significant potential in mitigating diseases related to oxidative stress. Mitochondria are the organelles most susceptible to molecular damage caused by oxidative stress, transcriptional pathways regulated by Nrf2 and PGC-1 play the crucial role in maintaining mitochondrial function and biogenesis. In this review the molecular mechanism of astaxanthin influence on Nrf2 and PGC-1α pathways and cellular health are analysed.
How the brain makes sense of the world: May-Britt Moser and the 2014 Nobel prize in Physiology or Medicine
T. V. Danylova1*, S. V. Komisarenko2
1Institute for Social and Political Psychology, National Academy
of Educational Sciences of Ukraine, Kyiv;
*e-mail: danilova_tv@ukr.net;
2Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv
Received: 21 April 2025; Revised: 27 May 2025;
Accepted: 11 June 2025; Available on-line: 07 July 2025
For hundreds of years, thinkers have tried to unravel the mystery of the brain and the ways the brain makes sense of the world. In recent decades, prominent neuroscientists have come close to solving this phenomenon and have provided crucial information about the role of the brain in complex behavior. One of them is May-Britt Moser, a Norwegian psychologist and neuroscientist known for her work on spatial orientation and spatial memory specifically and cognition more generally and a co-recipient of the 2014 Nobel Prize in Physiology or Medicine. This paper aims to outline the main stages of her scientific activities.
Corrigendum: Interaction of 4 allotropic modifications of carbon nanoparticles with living tissues
Paryzhak S.Ya., Dumych T.I., Peshkova S.M.,
Bila E.E., Lutsyk A.D., Barras A.,
Boukherroub R., Szunerits S., Bilyy R.O.
Ukr Biochem J. 2019; 91(2): 41-50.
doi: https://doi.org/10.15407/ubj91.02.041
In the published article, there was an error in Figure 5 as published. Figure 5 included by mistake a wrong fluorescence image of mice used in this work. We believe this error resulted from the use of the same contour mask during figure preparation, inadvertently leading to a mix-up of images.
The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way.
The updated Figure 5 is shown below.
Isolation, characterization and antioxidant activity of fibrinogen-like protein-1 from serum and synovial fluid of patients with rheumatoid arthritis
Abdulsattar J. Abdullah, Zahraa M. A. Hamodat*
Department of Chemistry, College of Science, University of Mosul, Iraq;
*e-mail: zahraahamodat@uomosul.edu.iq
Received: 20 April 2025; Revised: 01 June 2025;
Accepted: 11 June 2025; Available on-line: 07 July 2025
Rheumatoid arthritis (RA) is an autoimmune disease characterized by chronic inflammation and oxidative stress. Fibrinogen-like protein-1 (FGL1) has been implicated in immune regulation, but its antioxidant role under inflammatory conditions remains underexplored. This study aimed to isolate and purify FGL1 from the serum of healthy controls and from the serum and synovial fluid from inflamed joints of RA patients, and to assess its antioxidant capacity. Purification included ammonium sulfate precipitation (65%), dialysis, and gel filtration chromatography (Sephadex G-75), SDS-PAGE and HPLC. Antioxidant activity was evaluated by DPPH radical scavenging and IC50 calculation. SDS-PAGE and HPLC analysis confirmed the successful isolation, identity and high purity of FGL1 from all samples, the protein molecular weight ranged from 68 to 70 kDa. The DPPH assay showed that FGL1 isolated from synovial fluid of RA patients had the highest antioxidant activity (IC50 = 2.124 ng/ml), followed by RA serum (2.172 ng/ml) and control serum (2.798 ng/ml). These results indicate the dual role of FGL1 protein in immune response and oxidative balance, making it a promising biomarker and potential therapeutic target in rheumatoid arthritis.