Tag Archives: C60 fullerene

Biochemical and tensometric analysis of C(60) fullerenes protective effect on the development of skeletal muscle fatigue

D. M. Nozdrenko1, K. I. Bogutska1, I. V. Pampuha1,
O. O. Gonchar2, O. M. Abramchuk3, Yu. I. Prylutskyy1*

1Taras Shevchenko National University of Kyiv, Ukraine;
*e-mail: prylut@ukr.net;
2Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv;
3Lesya Ukrainka Volyn National University, Lutsk, Ukraine

Received: 19 May 2021; Accepted: 07 July 2021

TThe protective effect of water-soluble C60 fullerenes on the development of slow and rapid fatigue of rat skeletal muscles was analyzed. It was found that the reduction of muscle contraction force (muscle soleus) by 50% of the initial values is almost twice as slow as stimulation with a frequency of 1 Hz (slow muscle fatigue) than with 2 Hz (rapid muscle fatigue) stimulation after intramuscular injection of C60  fullerenes (dose 0.5 mg/kg). There is a clear tendency to decrease the values of biochemical parameters of the blood of animals with the therapeutic effect of water-soluble C60 fullerenes by approximately 45-60% and 35-40% with the development of slow and rapid muscle fatigue, respectively. Thus, C60 fullerenes, as powerful antioxidants, are able to efficiently affect the prooxidant-antioxidant homeostasis of muscle tissue and thus help maintain its normal physiological state.

Influence of C(60) fullerene on the ischemia-reperfusion injury in the skeletal muscle of rat limb: mechanokinetic and biochemical analysis

D. O. Zavodovskyi1, S. Yu. Zay2, T. Yu. Matvienko1, Yu. I. Prylutskyy1,
N. Y. Nurishchenko1, S. S. Paradizova3, L. L. Bezuh3, U. Ritter4, P. Scharff4

1ESC Institute of Biology and Medicine, Taras Shevchenko National University of Kyiv, Ukraine;
2Lesya Ukrainka Eastern European National University, Lutsk, Ukraine;
3SI The Territorial Medical Association of the Ministry of Internal Affairs of Ukraine in Kyiv;
4Institute of Chemistry and Biotechnology, Technical University of Ilmenau, Ilmenau, Germany;
e-mail: Lab@univ.kiev.ua

Influence of the pristine C60 fullerene aqueous colloidal solution (C60FAS) on the ischemia-reperfusion injury in the skeletal muscle of rat limb was studied. Skeletal muscle damage effects were induced by 3 h lasting­ vascular ischemia. The impact of C60FAS was studied after its intramuscular injection immediately after 1 h of reper­fusion at different doses, namely: 1, 2 and 3 mg/kg of body weight. Changes in the mechanokinetic parame­ters of ischemic skeletal muscle contraction at different modes of functioning and biochemical parame­ters of blood were used as markers of ischemic injury, and analyzed in detail under action of C60FAS. The obtained results indicate on great promise of use of C60FAS to reduce the consequences of ischemic muscle trauma.

C(60) fullerene prevents genotoxic effects of doxorubicin in human lymphocytes in vitro

K. S. Afanasieva1, S. V. Prylutska1, A. V. Lozovik1, K. I. Bogutska1, A. V. Sivolob1,
Yu. I. Prylutskyy1, U. Ritter2, P. Scharff2

1Taras Shevchenko National University of Kyiv, Ukraine;
е-mail: prylut@ukr.net;
2Technical University of Ilmenau, Institute of Chemistry
and Biotechnology, Ilmenau, Germany 

The self-ordering of C60  fullerene, doxorubicin and their mixture precipitated from aqueous solutions was investigated using atomic-force microscopy. The results suggest the complexation between the two compounds. The genotoxicity of doxorubicin in complex with C60  fullerene (С60+Dox) was evaluated in vitro with comet assay using human lymphocytes. The obtained results show that the C60  fullerene prevents the toxic effect of Dox in normal cells and, thus, С60+Dox complex might be proposed for biomedical application.