Tag Archives: C60 fullerene
C(60) fullerene restores gastrocnemius contractile activity in a rat model of neurogenic muscle atrophy
D. M. Nozdrenko1, M. S. Anhelov1, T. Yu. Matviienko1,
O. V. Lynchak1, O. V. Korzhyk2, Yu. M. Valetskyi3,
K. I. Bogutska1, Yu. I. Prylutskyy1*
1Taras Shevchenko National University of Kyiv, Kyiv, Ukraine;
*e-mail: prylut@ukr.net;
2Lesya Ukrainka Volyn National University, Lutsk, Ukraine;
3Municipal Institution of Higher Education
“Volyn Medical Institute”, Lutsk, Ukraine
Received: 11 March 2025; Revised: 27 March 2025;
Accepted: 25 April 2025; Available on-line: 12 May 2025
Restoration of the motor function of the musculoskeletal system impaired due to innervation damage is an important clinical problem. In the study the potential therapeutic effect of C60 fullerene application was estimated in the Wistar rat model of neurogenic muscle atrophy caused by nervus ischiadicus injury. The animals were divided into the following: control, injury, injury+С60 groups. C60 fullerene aqueous solution was administrated orally for 30 days after ischiadicus injury at a daily dose of 1 mg/kg. Biomechanical parameters of gastrocnemius muscle contraction and biochemical indices (creatinine, lactate, reduced glutathione content as well as creatine phosphokinase, lactate dehydrogenase, catalase and superoxide dismutase activity) in the blood of rats were estimated on day 30 after nerve transection. It has been found that muscle strength response in the injury+С60 group was significantly enhanced, in particular, the muscle force impulse was increased by more than 30 ± 2% compared to the injury group. The studied biochemical indices of the muscle fatigue and oxidative stress in the blood of experimental animals had a pronounced tendency to increase after the initiation of the muscle neurogenic atrophy, while under the influence of C60 fullerene they decreased compared with the injury group. In our opinion, C60 fullerene prevented significant dysfunction of the gastrocnemius muscle after neurogenic atrophy by exerting an antioxidant effect and improving its contractile activity.
C(60) fullerene effect on the functional activity of rat gastrocnemius muscle during its regeneration after the open injury
D. M. Nozdrenko1, O. O. Gonchar2, N. E. Nurishchenko1,
V. O. Stetska1, T. Yu. Matviienko1, Ya. V. Stepanyuk3,
K. I. Bogutska1, Yu. I. Prylutskyy1*
1ESC “Institute of Biology and Medicine”, Taras Shevchenko National University of Kyiv, Ukraine;
2Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv;
3Medical Faculty, Lesya Ukrainka Volyn National University, Lutsk, Ukraine;
*e-mail: prylut@ukr.net
Received: 05 September 2024; Revised: 14 October 2024;
Accepted: 21 November 2024; Available on-line: 17 December 2024
Open injuries are one of the most common skeletal muscle traumas. The study aimed to estimate the effect of the oral administration of C60 fullerene aqueous solution (C60FAS) daily at a dose of 1 mg/kg on the restoration of rat skeletal muscle functional activity on the 5th, 10th and 15th day after the open trauma. Male Wistar rats were randomly divided into three groups of 12 animals in each: control, with muscle injury and with muscle injury+C60FAS. The isolated gastrocnemius muscle was subjected to open injury by transverse dissection with a depth of 1 mm. Stimulation of muscle efferents was carried out by electrical impulses generated using a strain gauge generator. The content of C-reactive protein, creatinine, lactate, reduced glutathione and the activity of catalase and superoxide dismutase in the rat blood were determined. According to the data obtained, application of C60FAS promotes the restoration of the functional activity of injured muscle, which was confirmed by a significant increase in gastrocnemius muscle force impulse, attenuation of the inflammatory and development of fatigue and normalization of pro- and antioxidant balance in the process of regeneration.
C(60) fullerene attenuates the signs of acute renal failure in rats under rhabdomyolysis due to inhibition of oxidative stress
O. Omelchuk1, S. Prylutska2, D. Nozdrenko3,
O. Motuziuk1,3, I. Vareniuk3, K. Bogutska3,
O. Vygovska4, А. Zholos3, Yu. Prylutskyy3*
1Lesya Ukrainka Volyn National University, Lutsk, Ukraine;
2National University of Life and Environmental Science of Ukraine, Kyiv, Ukraine;
3Taras Shevchenko National University of Kyiv, Ukraine;
*e-mail: prylut@ukr.net;
4Bogomolets National Medical University, Kyiv, Ukraine
Received: 14 August 2023; Revised: 18 September 2023;
Accepted: 27 October 2023; Available on-line: 06 November 2023
Rhabdomyolysis, as an acute stage of myopathy is known to be associated with the accumulation of muscle breakdown products, acute renal failure and oxidative stress. The goal of the study was to evaluate the effect of C60 fullerene as an antioxidant on kidney damage in the model of glycerol-induced rhabdomyolysis in rats. The study was conducted on male Wistar rats, divided into the following experimental groups: control animals, animals intramuscularly injected with glycerol in a doses of 5, 10 and 15 mg/kg and those intraperitoneally injected daily with C60 fullerene aqueous solution (C60FAS) in a dose of 1 or 2 mg/kg at 48 h after glycerol administration. Monitoring of the biochemical and morphological indicators was carried out on 3rd, 6th and 9th days of the experiment. A close correlation between the acute renal damage severity, increased creatinine and urea level, superoxide dismutase (SOD) and catalase (CAT) activity in the blood of rats was observed. It was shown that in rats which received 2 mg/kg of C60FAS the renal glomeruli size and necrosis manifestations were attenuated, whereas SOD and CAT activity in the blood was significantly decreased. The results obtained may be useful for developing approaches to the treatment of pathological conditions of the muscular system caused by rhabdomyolysis and associated oxidative stress.
Biochemical parameters of blood and tissue of the gastrocnemius muscle in chronically alcoholized rats under oral administration of C(60) fullerene aqueous solution
O. Motuziuk1,2, D. Nozdrenko2, S. Prylutska3,
K. Bogutska2, O. Korotkyi2, Yu. Prylutskyy2*
1Lesya Ukrainka Volyn National University, Lutsk, Ukraine;
2Taras Shevchenko National University of Kyiv, Ukraine;
*e-mail: prylut@ukr.net;
3National University of Life and Environmental Science of Ukraine, Kyiv
Received: 17 March 2023; Revised: 01 May 2023;
Accepted: 05 June 2023; Available on-line: 20 June 2023
Biochemical indices of blood and tissue of the gastrocnemius muscle chronically alcoholized (for 3, 6 and 9 months) rats were studied. С60 fullerene aqueous solution (C60FAS) was administered orally as a pharmacological agent at a dose of 1 mg/kg daily throughout the experiment in a three routes: 1 h before alcohol intake (preventive regimen), together with alcohol (therapeutic regimen I) and 1 h after alcohol intake (therapeutic regimen II). Creatine phosphokinase (CPK), lactate dehydrogenase (LDH), catalase, superoxide dismutase, glutathione peroxidase (GPx) activity and the level of creatinine, lactate, hydrogen peroxide, reduced glutathione were estimated with clinical diagnostic kits. A pronounced upward trend in creatinine and lactate content, CPK and LDH activity with increasing degree of alcoholic myopathy during experiment was detected. Administration of C60FAS was shown to reduce the biochemical indices of muscle injury and to reduce oxidative processes by maintaining the balance between pro-oxidant and antioxidant systems. The maximum positive effect was observed when C60FAS was administered together with alcohol (therapeutic regimen I). The results indicate on C60 fullerene ability to correct the pathological condition of the muscular system arising from alcohol intoxication.
Biochemical and tensometric analysis of C(60) fullerenes protective effect on the development of skeletal muscle fatigue
D. M. Nozdrenko1, K. I. Bogutska1, I. V. Pampuha1,
O. O. Gonchar2, O. M. Abramchuk3, Yu. I. Prylutskyy1*
1Taras Shevchenko National University of Kyiv, Ukraine;
*e-mail: prylut@ukr.net;
2Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv;
3Lesya Ukrainka Volyn National University, Lutsk, Ukraine
Received: 19 May 2021; Accepted: 07 July 2021
TThe protective effect of water-soluble C60 fullerenes on the development of slow and rapid fatigue of rat skeletal muscles was analyzed. It was found that the reduction of muscle contraction force (muscle soleus) by 50% of the initial values is almost twice as slow as stimulation with a frequency of 1 Hz (slow muscle fatigue) than with 2 Hz (rapid muscle fatigue) stimulation after intramuscular injection of C60 fullerenes (dose 0.5 mg/kg). There is a clear tendency to decrease the values of biochemical parameters of the blood of animals with the therapeutic effect of water-soluble C60 fullerenes by approximately 45-60% and 35-40% with the development of slow and rapid muscle fatigue, respectively. Thus, C60 fullerenes, as powerful antioxidants, are able to efficiently affect the prooxidant-antioxidant homeostasis of muscle tissue and thus help maintain its normal physiological state.
Influence of C(60) fullerene on the ischemia-reperfusion injury in the skeletal muscle of rat limb: mechanokinetic and biochemical analysis
D. O. Zavodovskyi1, S. Yu. Zay2, T. Yu. Matvienko1, Yu. I. Prylutskyy1,
N. Y. Nurishchenko1, S. S. Paradizova3, L. L. Bezuh3, U. Ritter4, P. Scharff4
1ESC Institute of Biology and Medicine, Taras Shevchenko National University of Kyiv, Ukraine;
2Lesya Ukrainka Eastern European National University, Lutsk, Ukraine;
3SI The Territorial Medical Association of the Ministry of Internal Affairs of Ukraine in Kyiv;
4Institute of Chemistry and Biotechnology, Technical University of Ilmenau, Ilmenau, Germany;
e-mail: Lab@univ.kiev.ua
Influence of the pristine C60 fullerene aqueous colloidal solution (C60FAS) on the ischemia-reperfusion injury in the skeletal muscle of rat limb was studied. Skeletal muscle damage effects were induced by 3 h lasting vascular ischemia. The impact of C60FAS was studied after its intramuscular injection immediately after 1 h of reperfusion at different doses, namely: 1, 2 and 3 mg/kg of body weight. Changes in the mechanokinetic parameters of ischemic skeletal muscle contraction at different modes of functioning and biochemical parameters of blood were used as markers of ischemic injury, and analyzed in detail under action of C60FAS. The obtained results indicate on great promise of use of C60FAS to reduce the consequences of ischemic muscle trauma.
C(60) fullerene prevents genotoxic effects of doxorubicin in human lymphocytes in vitro
K. S. Afanasieva1, S. V. Prylutska1, A. V. Lozovik1, K. I. Bogutska1, A. V. Sivolob1,
Yu. I. Prylutskyy1, U. Ritter2, P. Scharff2
1Taras Shevchenko National University of Kyiv, Ukraine;
е-mail: prylut@ukr.net;
2Technical University of Ilmenau, Institute of Chemistry
and Biotechnology, Ilmenau, Germany
The self-ordering of C60 fullerene, doxorubicin and their mixture precipitated from aqueous solutions was investigated using atomic-force microscopy. The results suggest the complexation between the two compounds. The genotoxicity of doxorubicin in complex with C60 fullerene (С60+Dox) was evaluated in vitro with comet assay using human lymphocytes. The obtained results show that the C60 fullerene prevents the toxic effect of Dox in normal cells and, thus, С60+Dox complex might be proposed for biomedical application.