Tag Archives: rats
Effects of ethylthiosulfanylate and chromium (VI) on the state of pro/antioxidant system in rat liver
B. І. Kotyk1, R. Ya. Iskra1, O. M. Slivinska1, N. M. Liubas1,
A. Z. Pylypets1, V. I. Lubenets2, V. I. Pryimych3
1Institute of Animal Biology, NAAS of Ukraine, Lviv;
2Lviv Polytechnic National University, Ukraine;
3Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv, Ukraine;
e-mail: kicyniabo@gmail.com
Received: 04 April 2020; Accepted: 25 June 2020
Ethylthiosulfanylate is alkyl ester of thiosulfoacid and belongs to the class of thiosulfonate compounds. Structurally, thiosulfonates are synthetic analogues of natural phytoncides. It is known that, natural organic sulfur-containing compounds are characterized by antioxidant and detoxification properties against heavy metals toxicity. Therefore, the purpose of the study was to investigate the influence of ethylthiosulfanylate, as a synthetic analogue of natural phytoncides, on the state of the pro/antioxidant system in the liver of laboratory rats exposed to Cr(VI). It was found that ethylthiosulfanylate exposure at a dose 100 mg/kg body weight daily for 14 days led to a decrease in the intensity of increasing of the lipid hydroperoxides (LHP) content in the rat liver caused by Cr(VI) action. In addition, ethylthiosulfanylate pretreatment prevented depletion of reduced glutathione (GSH) pool under the action of potassium dichromate oxidative stress and performed the accumulation of cellular GSH in rat liver.
The indices of nitrogen (II) oxide system in experimental hepatopulmonary syndrome
I. Ya. Krynytska, M. I. Marushchak
I. Horbachevsky Ternopil State Medical University, Ternopil, Ukraine;
e-mail: marushchak@tdmu.edu.ua
Hepatopulmonary syndrome (HPS) is a pulmonary complication of liver disease characterized by arterial hypoxemia. Altered nitrogen (II) oxide (NO) production has also been implicated in the pathogenesis of the HPS. The present study was designed to evaluate the indices of NO system in the blood serum and lung tissue of animals with different models of hepatopulmonary syndrome. The total NOS activity was performed by monitoring the rate of conversion of L-arginine into citrulline. The total contents of NO metabolites was assessed by evaluation of their amount, which included nitrite ions that were previously presented in the sample (NO2–) and also nitrate ions reducted to nitrites (NO3–). We found a significant increase in total NOS activity in the lung tissue of Rats of both experimental groups as compared to control animals, but it was greater in the rats on the 28th day after the common bile duct ligation. The total concentration of NO2– + NO3– in the lung tissue of the rats in the experimental group N 1 also significantly increased (5.8 times) and in the rats of the experimental group with carbon – 4.5 times (P < 0.001) vs the control group. Thus, in rats with different models of hepatopulmonary syndrome the activation of nitroxydergic process by a significant increase in nitrogen (II) oxide metabolites contents and total NO synthases activity has been established. Herewith a more pronounced intensification of nitroxydergic processes was observed in rats on the 28th day after the common bile duct ligation.
Effects of L-glutamic acid and pyridoxine on glutathione depletion and lipid peroxidation generated by epinephrine-induced stress in rats
N. O. Salyha
Institute of Animal Biology, NAAS of Ukraine, Lviv;
е-mail: ynosyt@yahoo.com
The main goal of this research was to investigate and compare the protective effects of L-glutamic acid (L-Glu) alone and combined with Pyridoxine (L-Glu+Pyridoxine) for the purpose of suppression and mitigation of epinephrine-induced stress in rats. This study outlines possible links between changes of reduced glutathione (GSH) level, antioxidant enzymes activity and content of the lipid peroxidation products after administration of the above-mentioned substances and under the action of stress in various tissues of rats. The obtained results suggest that the GSH level was significantly inhibited by stress in all investigated tissues (except kidneys). We have shown that under the stress, activities of glutathione-associated enzymes were changed (mainly decreased) in all investigated tissues. In rats, additionally received L-Glu and L-Glu+Pyridoxine, much less changes or lack of changes in studied parameters were observed. The content of lipid peroxidation products (lipid peroxides (LOOH) and thiobarbituric acid reactive substances (TBARS)) in myocardium, liver and kidney tissues of experimental groups under the stress conditions were significantly higher compared to the control. While in experimental groups that received L-Glu and L-Glu+Pyridoxine LOOH content in kidney, spleen and liver and TBARS content in spleen, liver and myocardium were almost at the level of control values. These results indicate that L-Glu and L-Glu+Pyridoxine can mitigate and suppress epinephrine-induced stress in rats.
Changes in glutathione system and lipid peroxidation in rat blood during the first hour after chlorpyrifos exposure
V. P. Rosalovsky, S. V. Grabovska, Yu. T. Salyha
Institute of Animal Biology, National Academy of Agrarian Sciences of Ukraine, Lviv;
e-mail: ros.volodymyr@gmail.com
Chlorpyrifos (CPF) is a highly toxic organophosphate compound, widely used as an active substance of many insecticides. Along with the anticholinesterase action, CPF may affect other biochemical mechanisms, particularly through disrupting pro- and antioxidant balance and inducing free-radical oxidative stress. Origins and occurrence of these phenomena are still not fully understood. The aim of our work was to investigate the effects of chlorpyrifos on key parameters of glutathione system and on lipid peroxidation in rat blood in the time dynamics during one hour after exposure. We found that a single exposure to 50 mg/kg chlorpyrifos caused a linear decrease in butyryl cholinesterase activity, increased activity of glutathione peroxidase and glutathione reductase, alterations in the levels of glutathione, TBA-active products and lipid hydroperoxides during 1 hour after poisoning. The most significant changes in studied parameters were detected at the 15-30th minutes after chlorpyrifos exposure.
Biochemical indicators of hepatotoxicity in blood serum of rats under the effect of novel 4-thiazolidinone derivatives and doxorubicin and their complexes with polyethyleneglycol-containing nanoscale polymeric carrier
L. I. Kоbylinska1, D. Ya. Havrylyuk1, А. О. Ryabtseva2, N. E. Mitina2,
О. S. Zаichenko2, R. B. Lesyk1, B. S. Zіmenkovsky1, R. S. Stoika3
1Danylo Halytsky Lviv National Medical University, Ukraine;
e-mail: lesya8@gmail.com;
2Lviv Polytechnic National University, Ukraine;
3Іnstitute of Cell Biology, National Academy of Sciences of Ukraine, Lviv
The aim of this study was to compare the effect of new synthetic 4-tiazolidinone derivatives (compounds 3882, 3288 and 3833) and doxorubicin (positive control) in free form and in their complexes with synthetic polyethyleneglycol-containing nanoscale polymeric carrier on the biochemical indicators of hepatotoxicity in blood serum of rats. The activity of enzymes considered as the markers of hepatotoxicity, as well as the concentration of total protein, urea and creatinine were measured in blood serum of rats. It was found that after injection of investigated compounds the activities of alanine aminotransferase, alkaline phosphatase and α-amylase increased in comparison to control. Doxorubicin injection was accompanied by 4-fold increase in the activity of γ-glutamyltransferase, and injection of compound 3833 led to 2.5-fold elevation of the activity of this enzyme. Complexation of these аntineoplastic derivatives with a synthetic nanocarrier lowered the activity of the investigated enzymes substantially if compared to the effect of these compounds in free form. The most evident decrease was measured for α-amylase, γ-glutamyltransferase and lactate dehydrogenase activities. The normalization of concentrations of total protein, urea and creatinine in blood serum of rats treated with complexes of the studied compounds with a polymeric carrier comparing with their introduction in free form was also detected. Thus, the immobilization by novel polymeric carrier of anticancer drugs possessing high general toxicity in the treated organism mitigates their toxic effect, which is evident as normalization of specific biochemical indicators of the hepatodestructive effects of the anticancer drugs.