Ukr.Biochem.J. 2014; Volume 86, Issue 6, Nov-Dec, pp. 56-65


Antioxidant defense system state in blood plasma and heart muscle of rats under the influence of histamine and sodium hypoclorite

O. I. Bishko, N. P. Harasym, D. I. Sanahurs’kyj

Ivan Franko National University of Lviv, Ukraine;

There is a wide spectrum of antihistamine drugs in the pharmaceutical market, however all these chemical preparations cause side effects. Therefore, new alternative ways for histamine detoxication are to be found. For this aim in our experiment sodium hypochlorite was used because its solution possesses strong oxidizing properties. The influence of histamine and sodium hypochlorite on the antioxidant defence system state of blood plasma and cardiac muscle in rats has been researched. It was shown, that the investigated factors result in the disruption of the antioxidant system. It was found that histamine injection in concentration of 1 and 8 μg/kg in plasma leads to the increase of superoxi­de dismutase activity during all the experiment. When studying enzymes, that catalyze hydroperoxides and Н2О2 decomposition it was shown that under the influence of histamine in a dose 1 μg/kg, the glutathione peroxidase activity increased on the 1st day of the experiment. However, on the 7th day of the experiment the increase of both glutathione peroxidase and catalase activity was fixed. The deviation in superoxide dismutase function in rats plasma under the action of sodium hypochlorite has been established. The activity of enzymes that decompose Н2О2 and hydroperoxides were inhibi­ted. Under the influence of histamine in the heart tissues we have stated the disturbance of superoxide dismutase work and increase of catalase activity and decrease of glutathione peroxidase activity. The influence of sodium hypochlorite on the myocardium of intact animals as well as joint influence of sodium hypochlorite and histamine result in the increase of superoxide dismutase and catalase activity and lead to the conside­rable decline of activity of glutathione peroxidase.

Keywords: , , , , , , ,


  1. Shahid M, Tripathi T, Sobia F, Moin S, Siddiqui M, Khan RA. Histamine, histamine receptors and their role in immunomodulation: an updated systematic. Open Immunol. J. 2009 Mar;2(1):9-41.  CrossRef
  2. Kaliner MA, Berger WE, Ratner PH, Siegel CJ. The efficacy of intranasal antihistamines in the treatment of allergic rhinitis. Ann Allergy Asthma Immunol. 2011 Feb;106(2 Suppl):S6-S11. Review. PubMed, CrossRef
  3. Menshchikova EB, Lankin VZ, Zenkov NK, Bondar IA, Krugovykh NF, Trufakin VA. Oxidative stress. Prooxidants and antioxidants. M: Word, 2006. 556 p. (In Russian).
  4. Golovchak NP. Free radical processes in the cardiac muscle of poultry for the actions of sodium hypochlorite. Visnyk of the Lviv University. Series Biology. 2013;62:29-37. (In Ukrainian).
  5. Kushnir G. V. Pharmacological action high-purity sodium hypochlorite on animals for chronic T-2 toxicity. Author. thesis. cand. wet. science. L., 2009. 22 р.
  6. Aubut V, Pommel L, Verhille B, Orsière T, Garcia S, About I, Camps J. Biological properties of a neutralized 2.5% sodium hypoclorite solution. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2010 Feb;109(2):e120-5. PubMed, CrossRef
  7. Komarenko VI, Terekhov AA, Voro­byova AP, Yanchuk PI. Investigation of the role of H1-receptor responses in rat liver portal vessels to histamine. Cherk Nat Univ B. Khmelnitsky. Biol. Sci. Series. 2008; 128:54-58. (In Ukrainian).
  8. Nesterova LA, Smurova EA, Manukhin BN. Characterization of specific binding blocker [+H]-quinuclidinylbenzilate M-cholinergic receptors in rat brain cortex membranes. Rep Acad Sci. 1995;343(2):268-271. (In Russian).
  9. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265-75. PubMed
  10. Kostiuk VA, Potapovich AI, Kovalevа ZhV. A simple and sensitive method of determination of superoxide dismutase activity based on the reaction of quercetin oxidation.  Vopr Med Khim. 1990 Mar-Apr;36(2):88-91. Russian. PubMed
  11. Koroljuk M. A., Ivanovа L. I., Mayorov I. G. Method for determination of catalase activity. Labor Work. 1988;1:16-19. (In Russian).
  12. Moin VM. A simple and specific method for determining glutathione peroxidase activity in erythrocytes. Lab Delo. 1986;(12):724-7. Russian. PubMed
  13. Bishko O., Golovchak N., Sanagurski D. Effect of histamine and sodium hypochlorite on free radical processes in lung tissues of rats. Studiа Biologica. 2013;7(3):97-106. (In Ukrainian).
  14. Dubinin EE. Рroducts of oxygen metabolism in the functional activity of cells (life and death, creation and destruction). Physiological and clinical-biochemical aspects. M. :SРb. Med. Press, 2006. 400 р. (In Russian).
  15. Kotsyumbas I, Velichenko O, Kotsyumbas G. Prospects of application hypochlorite in veterinary medicine. L.: TzOB VF “Afisha”, 2009. 312 p. (In Ukrainian).
  16. Golovchak NP, Tarnovska AV, Sanagurski D I. Lipid peroxidation in living organisms. L: Ivan Franko National University of Lviv, 2012. 250 p. (In Ukrainian).
  17. Victorov A. Adverse effects of modern medicines antihistamines. Modern Problems of Toxicology – 2003. Regime of access: http:. arhiv_mg. 1_2003.htm.
  18. Kitbunnadaj R. Histamine Receptors and Their Ligands. Naresuan Univ. J. 2005;13:41-53.
  19. Golovchak N, Tarnovska A, Bura M, Romaniuk M. Рrooxidant-antioxidant homeo­stasis loach embryos under the action of low-intensity laser irradiation. Phys Alive. 2009;17:76-81. (In Ukrainian).

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License.