Ukr.Biochem.J. 2021; Volume 93, Issue 3, May-Jun, pp. 68-74

doi: https://doi.org/10.15407/ubj93.03.068

Oxidative stress in the heart of rats exposed to acute intermittent hypobaric hypoxia

S. Dewi1*, M. Sadikin1, W. Mulyawan2

1Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia;
2Department of Aerophysiology, Lakespra Saryanto, Air Force Indonesian National Army, Jakarta, Indonesia;
*e-mail: syarifah.dewi@ui.ac.id

Received: 01 October 2020; Accepted: 17 May 2021

It is known that the altitude area causes hypoxic conditions due to the low oxygen partial pressure. This study was conducted to estimate oxidative stress indices in the heart tissue after Wister rats exposure to the acute intermittent hypobaric hypoxia. Hypobaric hypoxia exposure was simulated by keeping the rats in a hypobaric chamber for 1 min at 35,000 feet altitude. After that the altitude was gradually reduced to 30,000 and 25,000 feet and maitained for 5 min. 25 male Wistar rats were divided into control group and four treatment groups (I-IV), consisting of rats exposed 1, 2, 3 and 4 times to hypobaric hypoxia with a frequency once a week. The animals were removed from the experiment at the  height of 18,000 feet and the heart tissue was obtained. The carbonyl groups and  MDA levels and superoxide dismutase and  catalase activity were exami­ned in the supernatant of the heart tissue homogenate. In the samples of group I, the decrease  in catalase activity with a simultaneous notable increase in carbonyl groups level was observed compared to control. In the samples of groups III and IV, the carbonyl level normalized and the activity of  both antioxidant enzymes increased significantly. It was concluded that the increase of antioxidant enzymes activity can contribute to cardiac tissue adaptive response to acute hypobaric hypoxia exposure.

Keywords: , , ,


References:

  1. Donegani E, Hillebrandt D, Windsor J, Gieseler U, Rodway G, Schöffl V, Küpper T. Pre-existing cardiovascular conditions and high altitude travel. Travel Med Infect Dis. 2014;12(3):237-252. PubMedCrossRef
  2. Bärtsch P, Gibbs JSR. Effect of altitude on the heart and the lungs. Circulation. 2007;116(19):2191-2202. PubMed, CrossRef
  3. Bakonyi T, Radak Z. High altitude and free radicals. J Sports Sci Med. 2004;3(2):64-69. PubMed, PubMedCentral
  4. Ayala A, Muñoz MF, Argüelles S. Lipid peroxidation: production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Oxid Med Cell Longev. 2014;2014:360438. PubMed, PubMedCentral, CrossRef
  5. Grimsrud PA, Xie H, Griffin TJ, Bernlohr DA. Oxidative stress and covalent modification of protein with bioactive aldehydes. Biol Chem. 2008;283(32):21837-21841. PubMed, PubMedCentral, CrossRef
  6. McCord JM, Edeas MA. SOD, oxidative stress and human pathologies: a brief history and a future vision. Biomed Pharmacother. 2005;59(4):139-142. PubMed, CrossRef
  7. Al-Abrash AS, Al-Quobaili FA, Al-Akhras GN. Catalase evaluation in different human diseases associated with oxidative stress. Saudi Med J. 2000;21(9):826-830. PubMed
  8. Møller P, Loft S, Lundby C, Olsen NV. Acute hypoxia and hypoxic exercise induce DNA strand breaks and oxidative DNA damage in humans. FASEB J. 2001;15(7):1181-1186. PubMed, CrossRef
  9. Esteva S, Pedret R, Fort N, Torrella JR, Pagès T, Viscor G. Oxidative stress status in rats after intermittent exposure to hypobaric hypoxia. Wilderness Environ Med. 2010;21(4):325-331. PubMed, CrossRef
  10. Jefferson JA, Simoni J, Escudero E, Hurtado ME, Swenson ER, Wesson DE, Schreiner GF, Schoene RB, Johnson RJ, Hurtado A. Increased oxidative stress following acute and chronic high altitude exposure. High Alt Med Biol. 2004;5(1):61-69. PubMed, CrossRef
  11. Cmde A, Tyagi P, Cmde A, Malik H. Suggested operating schedules for sudden induction of unacclimatized or partially acclimatized aircrew for air operations at high altitude. Indian J Aerosp Med. 2008;52(2):9-14.
  12. Levine RL, Williams JA, Stadtman EP, Shacter E. Carbonyl assays for determination of oxidatively modified proteins. In: Methods in Enzymology. 1994:346-357. CrossRef
  13. George P. Reaction Between Catalase and Hydrogen Peroxide. Nature. 1947;160(4054):41-43.  CrossRef
  14. Birben E, Sahiner UM, Sackesen C, Erzurum S, Kalayci O. Oxidative stress and antioxidant defense. World Allergy Organ J. 2012;5(1):9-19. PubMed, PubMedCentral, CrossRef
  15. Bleier L, Dröse S.  Superoxide generation by complex III: from mechanistic rationales to functional consequences. Biochim Biophys Acta. 2013;1827(11-12):1320-1331. PubMed, CrossRef
  16. Magalhães J, Ascensão  A, Soares JMC, Ferreira R, Neuparth MJ, Marques F, Duarte JA. Acute and severe hypobaric hypoxia increases oxidative stress and impairs mitochondrial function in mouse skeletal muscle. J Appl Physiol. 2005;99(4):1247-1253. PubMed, CrossRef
  17. Dewi S, Mulyawan W, Wanandi SI, Sadikin M. The Effect of Intermittent Hypobaric Hypoxia on Oxidative Stress Status and Antioxidant Enzymes Activity in Rat Brain. Acta Biochim Indones. 2018;1(2):46-51. CrossRef
  18. Park AM, Suzuki YJ. Effects of intermittent hypoxia on oxidative stress-induced myocardial damage in mice. J Appl Physiol. 2007;102(5):1806-1814. PubMed, CrossRef

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License.