Category Archives: Uncategorized
Structural proteins in the mechanisms of betacoronavirus virion assembly
I. Zaloilo1*, О. Zaloilo2, Y. Rud3, L. Buchatskyi3
1National University of Life and Environmental Sciences of Ukraine, Kyiv;
2Institute of Fisheries of the National Academy of Agrarian Sciences of Ukraine, Kyiv;
3ESC Institute of Biology and Medicine, Taras Shevchenko National University of Kyiv, Ukraine;
*e-mail: zaloilo76@gmail.com, iridolpb@gmail.com
Received: 08 August 2022; Revised: 31 August 2022;
Accepted: 04 November 2022; Available on-line: 14 November 2022
The emergence of SARS-CoV-2 caused an urgent need to investigate the molecular mechanisms of its reproduction. However, the detailed step-by-step mechanism of SARS-CoV-2 virion assembly has not been described yet. In the presented review the data on the role of structural proteins in the efficient assembly of betacoronavirus particles are analyzed.
New formulation and activity of rhenium-platinum antitumor system
N. I. Shtemenko1,2*, D. E. Kytova1, O. V. Berzenina1,
O. I. Hrabovska1,3, A. V. Shtemenko1
1Ukrainian State University of Chemical Technology, Dnipro, Ukraine;
2Oles Honchar Dnipro National University, Ukraine;
3SI “Institute of Gastroenterology of the National Academy of Medical Sciences of Ukraine”, Dnipro, Ukraine;
*e-mail: n.shtemenko@ukr.net
Received: 16 November 2021; Revised: 10 January 2022; Accepted: 29 September 2022; Available on-line: October 2022
Two-component Rhenium-Platinum system (Re-Pt system) is based on administration of a cluster dirhenium(III) compound and cisplatin to tumor bearing animals followed by a significant antitumor effect and decreased toxic effect of cisplatin on normal cells. The aim of this work was to obtain solid lipid nanoparticles (SLN) from surface lipids (waxes) of Chelidonium majus L. (Papaveraceae) leaves and to estimate whether capsulation of dirhenium(III) as a component of the Re-Pt system into SLN will affect its antitumor activity and red blood cells (RBC) morphology in a rat model of Guerin’s carcinoma growth. Fourier-transform infrared spectroscopy, gas-liquid chromatography, microscopy, light scattering were used in the research. Solid lipid nanoparticles were obtained, characterized, loaded with cluster dirhenium(III) and being introduced together with cisplatin to rats with Guerin’s carcinoma resulted in RBC morphology preservation and a significant decrease in tumor weight. It was concluded that the lipid coating of the rhenium cluster compound did not reduce the antitumor effect of the Re-Pt system and protected RBC from toxic cisplatin influence. A new formulation of the Re-Pt system is proposed.
Decamethoxin virucidal activity: in vitro and in silico studies
I. V. Semenyuta1*, O. P. Trokhimenko2, I. V. Dziublyk2, S. O. Soloviov2,3,
V. V. Trokhymchuk2, O. L. Bororova4, D. M. Hodyna1,
M. P. Smetiukh3, O. K. Yakovenko5, L. О. Metelytsia1
1V. P. Kukhar Institute of Bioorganic Chemistry and Petrochemistry, National Academy of Sciences of Ukraine, Kyiv;
2Shupyk National Healthcare University of Ukraine, Kyiv;
3National Technical University of Ukraine “Igor Sikorsky Kyiv Polytechnic Institute”, Kyiv;
4F. G. Yanovsky Institute of Tuberculosis and Pulmonology, National Academy of Medical Sciences of Ukraine, Kyiv;
5Volyn Regional Clinical Hospital, Lutsk, Ukraine;
*e-mail: ivan@bpci.kiev.ua
Received: 09 June 2021; Revised: 11 September 2022;
Accepted: 29 September 2022; Available on-line: 06 October 2022
The data on the representative of decamethoxin short-term action on infectious bronchitis virus (IBV) strain H120 used as a human-safe model of SARS-CoV-2 virus are presented. The viral activity was estimated with the use of inverted microscope PrimoVert (Germany) by destructive effect on BHK21 fibroblastic cell line. In vitro results demonstrated that decamethoxin (100 μg/ml) completely inactivated IBV coronavirus strain at exposure of 30 sec and more. At the lowest decamethoxin exposure of 10 sec the antiseptic virucidal activity was 33% and 36% of control at 24 and 48 h of cultivation respectively. Molecular docking analysis indicated the significant similarity of IBV and SARS-CoV-2 main protease (Mpro) structure. Docking studies of decamethoxin interaction with IBV Mpro and SARS-CoV-2 Mpro active centers demonstrated the ligand-protein complexes formation with the estimated binding energy of -8.6, -8.4 kcal/mol and key amino acid residues ASN26, GLY141, GLU187, GLU164, THR24, THR25, ASN142, GLY143, CYS145, HIS164 and GLU166.
Synthesis of the novel cage amides and imides and evaluation of their antibacterial and antifungal activity
V. Palchykov1*, A. Gaponov1, N. Manko2,3, N. Finiuk2,
О. Novikevych4, O. Gromyko3, R. Stoika2, N. Pokhodylo3,4*
1Research Institute of Chemistry and Geology, Oles Honchar Dnipro National University, Ukraine;
2Institute of Cell Biology of National Academy of Sciences of Ukraine, Lviv;
3Ivan Franko National University of Lviv, Ukraine;
4Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv, Ukraine;
*e-mail: pokhodylo@gmail.com; palchikoff82@gmail.com
Received: 28 December 2021; Revised: 29 June 2022;
Accepted: 29 September 2022; Available on-line: 06 October 2022
Cage amides and imides bearing bicyclo[2.2.1]- and bicyclo[2.2.2]-subunits were synthesized and evaluated both for antimicrobial activity toward five key ESKAPE pathogenic bacteria: one Gram‐positive bacteria methicillin‐resistant Staphylococcus aureus (ATCC 43300), four Gram‐negative bacteria Escherichia coli (ATCC 25922), Klebsiella pneumoniae (ATCC 700603), Acinetobacter baumannii (ATCC 19606) and Pseudomonas aeruginosa (ATCC 27853) and for antifungal activity towards pathogenic fungal strains Candida albicans (ATCC 90028) and Cryptococcus neoformans var. Grubii (H99; ATCC 208821). Compound VP-4539 with bicyclo[2.2.2]octene motif demonstrated the highest cytotoxic activity towards C. neoformans, while human keratinocytes of HaCaT line, murine fibroblasts of Balb/c 3T3 line and mitogen-activated lymphocytes of peripheral human blood were found to be tolerant to its action. VP-4539 compound did not intercalate into salmon sperm DNA indicating that its cytotoxicity is not related to intercalation into nucleic acid.
The level pro-inflammatory and anti-inflammatory biomarkers in patients with chronic mechanical low back pain under pulse radiofrequency therapy
I. Tekin1, F. Kosova2*
1Manisa Celal Bayar University Medical Faculty, Department of Anesthesia and Reanimation, Algology, Manisa, Turkey;
2Manisa Celal Bayar University School of Vocational Health Service, Department of Medical Biochemistry, Manisa, Turkey;
*e-mail: fundakosova@gmail.com
Received: 02 June 2022; Revised: 25 July 2022;
Accepted: 29 September 2022; Available on-line: 06 October 2022
Low back pain is a frequent and recurrent condition, often with a non-specific cause. Conventional treatment methods are generally insufficient in the treatment of chronic low back pain. The aim of the study was to estimate the level of IFN, IL-1, IL-6 (proinflammatory), IL-10, IL-4 (anti-inflammatory) and VEGF proteins in the serum of patients with chronic mechanical low back pain under Pulse radiofrequency (PRF) therapy. The study was carried out on 40 patients 20-60 years old, diagnosed with chronic low back pain for at least 4 months, primary complaint on lumbosacral low back pain, pain intensity VAS (visual analog scale) score of 5 and above, not responding well to conservative treatment (analgesic drugs, physiotherapy, etc.). Therapeutic Radiofrequency applications were carried out with an RF generator (RFG 3C Plus, Radionics). Blood samples were taken 1 day before interventional treatment (control), then 1 day (group1) and 15 days (group 2) after. The serum level of IFN, IL-1, IL-6, IL-10, IL-4 and VEGF l was analyzed with ELISA test. It was shown that as a result of PRF treatment the level of IL-1 was decreased while the levels of IL-4 and IL-6 were increased. It was concluded that the increase in serum levels of proinflammatory cytokines may be correlated with the severity of pain and that the increase in the level of anti-inflammatory cytokines reduces pain by reducing inflammation.
Dynamics of homocysteine level in patients with osteoporotic fracture
N. A. Hasanova
Azerbaijan Medical University, Biochemical Department, Baku, Azerbaijan;
e-mail: hasanovanaila@yahoo.com
Received: 04 June 2022; Revised: 01 August 2022;
Accepted: 29 September 2022; Available on-line: 06 October 2022
The research was carried out in order to investigate the blood serum level of homocysteine (HCY) which is involved in bone metabolism and has prognostic significance in the monitoring of the regenerative processes in osteoporosis and osteoporotic fractures. The study was carried out on patients 45-83 years old divided into 3 groups: group I – 14 patients with osteoporosis confirmed by densitometry or X-ray examination, group II – 15 patients with non-osteoporosis fractures, group III – 25 patients with osteoporotic fractures. The control group consisted of practically healthy 14 people. In patients with various fractures osteosynthesis with Ilizarov apparatus or with metal plates was performed. After the operation, the patients were treated in an inpatient setting for a week, then sent for outpatient treatment and prescribed calcium and vitamin D supplements to accelerate the bone regeneration process. A blood sample was taken at 3 stages to monitor the dynamics of HCY level by Elisa test: on the 1st day before treatment, on the 10th day of treatment and 1 month after it. The results showed that on the 1st day before the treatment HCY concentration was statistically increased 2.7 times in group I, 5.6 times in group II, and 6.5 times in group III compared to the control group. In the month of recovery, a significant decrease in HCY level was observed in all treated groups but it still remained higher than in the control indicating the need to recommend additional therapeutic prescriptions.
Detection of unusual high molecular form of albumin in blood serum of COVID-19 patients
Yu. Kit1, M. Starykovych1, N. Manko1, S. Kannan2,
A. Orfin3, S. Souchelnytskyi4, R. Stoika1*
1Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv;
2College of Medicine, Qatar University, Doha, Qatar;
3Municipal Non-commercial Enterprise of Lviv Regional Council “Lviv Regional Infection Clinical Hospital”, Lviv, Ukraine;
4Oranta CancerDiagnostics AB, Uppsala, Sweden;
*e-mail: stoika.rostyslav@gmail.com
Received: 07 June 2022; Revised: 15 September 2022;
Accepted: 29 September 2022; Available on-line: 06 October 2022
Blood sera of 12 severe Covid-19 patients and 14 healthy human donors were subjected to original TCA-extraction/acetone-precipitation followed by SDS-PAAG electrophoresis and mass-spectrometry. 76 kDa protein was detected as one of the differentially expressed proteins in the samples of Covid-19 patients. This 76 kDa protein was identified with mass-spectrometry as human serum albumin. Such molecular form of albumin was absent in blood serum of healthy human donors. The potential ways of generation of the unusual form of human serum albumin and its probable diagnostic value were discussed.
Correlation between adiponectin level and obesity as a risk factor for allergy disease
M. Spasovska1*, T. K. Panovska2
1General Hospital, Ohrid, R.Macedonia;
2Faculty of Pharmacy, Ss. Cyril and Methodius University, Skopje, R. Macedonia;
*e-mail: spasovskamilena@yahoo.com
Received: 28 November 2021; Revised: 30 June 2022;
Accepted: 29 September 2022; Available on-line: 06 October 2022
Much research has focused on the connection between two inflammatory conditions, allergic reactions and obesity which has led to a focus on adiponectin, hormone with anti-inflammatory properties secreted by adipose tissue. The aim of this study was to determine the association of adiponectin with obesity, as a risk factor for the development of allergic condition in order to rationalize approach to its treatment. Research methods for inflammatory markers and biochemical parameters involve immunoassay technique. Statistical analysis was performed with Student’s t-test, Wilcoxon T-test and coefficient of correlation. The study included apparently healthy subjects and patients with allergy conditions with confirmed presence of specific IgE, classified into 2 groups according to their body mass index (BMI). The obtained data showed negative correlation (cor = – 0.6), between adiponectin levels and BMI values. Thus, decreased level of adiponectin is associated with increased BMI. The mean values of adiponectin in the studied population, with high statistical differences between the groups (19.1 ± 1.5; 17.7 ± 0.9), (18.8± 1.1; 16.6 ± 1.0) demonstrated the relationship between low adiponectin level and development of obesity, and what, in turn, increasd risk of developing allergic conditions. The assumption was made that adiponectin may be used as a sensitive biochemical marker for early diagnostics of allergic reactions.
Involvement of Cu-containing amine oxidases in the development of lung pathology in ovalbumin-induced bronchial asthma in guinea pigs
O. Hudkova1*, S. Luhovskyi2, L. Drobot1, N. Latyshko1
1Palladin Institute of Biochemistry, National Academy of Sсiences of Ukraine, Kyiv;
2Dmitry F. Chebotarev Institute of Gerontology, National Academy of Medical Sciences of Ukraine, Kyiv;
*e-mail: ogudkova@biochem.kiev.ua
Received: 03 August 2022; Revised: 15 September 2022;
Accepted: 29 September 2022; Available on-line: 06 October 2022
Bronchial asthma is developed as an immune response to allergen challenges accompanied by inflammation and fibrosis implicated in airway remodeling. To reveal the causative implication of Cu-containing amine oxidases, semicarbazide-sensitive amine oxidase (SSAO), diamine oxidase (DAO), and lysyl oxidase (LOX), in bronchial asthma development we used their irreversible inhibitor, semicarbazide, and ovalbumin-induced pathology in guinea pigs. Semicarbazide was introduced to asthmatic animals via drink or inhalation. At the 16th week after disease induction, the increase in the activity of pro-inflammatory SSAO and DAO in plasma (1.6 and 2 times, respectively) was observed. The introduction of semicarbazide to asthmatic animals via drink or inhalation significantly decreased activities of these enzymes compared to the untreated asthmatic animals. A considerable increase in IL-13 content and LOX activity in the lung tissue of asthmatic animals were observed that evidenced airway inflammation and pulmonary fibrosis development. The uptake of semicarbazide by guinea pigs with bronchial asthma led to normalization of LOX activity. Histological studies confirmed that semicarbazide attenuated morphopathological changes in the lungs of asthmatic animals. Thus, the data obtained indicate the direct participation of the studied enzymes in the progression of pathological processes in atopic bronchial asthma as well as the potential use of semicarbazide as a drug in complex anti-asthmatic therapy.
Ferric oxide nanoparticles administration suppresses isoniazid induced oxidative stress in the rat brain tissue
H. Faramarzi1, J. Saffari-Chaleshtori2, S. Zolghadri3,
M. Beheshtroo4, A. Faramarzi5, S. M. Shafiee4,6*
1Department of Community Medicine, Faculty of Medicine, Shiraz University of Medical Sciences, Iran;
2Clinical Biochemistry Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran;
3Department of Biology, Jahrom Branch, Islamic Azad University, Jahrom, Iran;
4Department of Biochemistry, Shiraz Branch, Islamic Azad University, Shiraz, Iran;
5Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran;
6Autophagy Research Center, Shiraz University of Medical Sciences, Shiraz Iran;
*e-mail: shafieem@sums.ac.ir
Received: 08 November 2021; Revised: 27 June 2021;
Accepted: 29 September 2022; Available on-line: 06 October 2022
Isoniazid is one of the anti-tuberculosis therapeutic agents capable of causing side effects such as oxidative stress, brain tissue damage and mental disorders. This study aimed to investigate the effect of ferric oxide (Fe2O3) nanoparticles administration on isoniazid-induced oxidative stress parameters in rat brain tissue. Forty adult male Wistar rats (200–250 g) were randomly divided into a group with no treatment as control and four experimental groups. Animals of experimental groups received intraperitoneally for 12 days daily saline, 50 mg/kg of isoniazid, 50 mg/kg of isoniazid and 0.2 or 0.4 mg/kg Fe2O3 nanoparticles accordingly. The activity of catalase (CAT), superoxide dismutase (SOD), glutathione-S-transferase (GST), the level of glutathione (GSH), malondialdehyde (MDA) and total protein were determined in brain tissue homogenates by spectrophotometric methods. It was shown that CAT and GST activities, as well as GSH and MDA levels in the brain tissue of animals in the isoniazid-treated group were increased compared with the control untreated group, while following the treatment with 0.2 or 0.4 mg/kg Fe2O3 nanoparticles the studied oxidative stress parameters returned to the control level (P < 0.05). No changes in SOD activity in any of the treated groups were observed compared to the control. This study showed that the administration of ferric oxide nanoparticles can suppress isoniazid-induced oxidative stress in the brain tissue of rats mentally damaged by isoniazid.